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@ARTICLE{Wnsche:136836,
      author       = {P. Wünsche$^*$ and E. Eckert$^*$ and T. Holland-Letz$^*$
                      and A. Paruzynski and A. Hotz-Wagenblatt$^*$ and R.
                      Fronza$^*$ and T. Rath$^*$ and I. Gil-Farina$^*$ and M.
                      Schmidt$^*$ and C. von Kalle$^*$ and C. Klein and C.
                      Ball$^*$ and F. Herbst$^*$ and H. Glimm$^*$},
      title        = {{M}apping {A}ctive {G}ene-{R}egulatory {R}egions in {H}uman
                      {R}epopulating {L}ong-{T}erm {HSC}s.},
      journal      = {Cell stem cell},
      volume       = {23},
      number       = {1},
      issn         = {1934-5909},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2018-01274},
      pages        = {132 - 146.e9},
      year         = {2018},
      abstract     = {Genes that regulate hematopoietic stem cell (HSC)
                      self-renewal, proliferation, and differentiation are tightly
                      controlled by regulatory regions. However, mapping such
                      regions relies on surface markers and immunophenotypic
                      definition of HSCs. Here, we use γ-retroviral integration
                      sites (γRV ISs) from a gene therapy trial for 10 patients
                      with Wiskott-Aldrich syndrome to mark active enhancers and
                      promoters in functionally defined long-term repopulating
                      HSCs. Integration site clusters showed the highest ATAC-seq
                      signals at HSC-specific peaks and strongly correlated with
                      hematopoietic risk variants. Tagged genes were significantly
                      enriched for HSC gene sets. We were able to map over 3,000
                      HSC regulatory regions in late-contributing HSCs, and we
                      used these data to identify miR-10a and miR-335 as two
                      miRNAs regulating early hematopoiesis. In this study, we
                      show that viral insertion sites can be used as molecular
                      tags to assess chromatin conformation on functionally
                      defined cell populations, thereby providing a genome-wide
                      resource for regulatory regions in human repopulating
                      long-term HSCs.},
      cin          = {B280 / G100 / C060 / W610 / L301},
      ddc          = {570},
      cid          = {I:(DE-He78)B280-20160331 / I:(DE-He78)G100-20160331 /
                      I:(DE-He78)C060-20160331 / I:(DE-He78)W610-20160331 /
                      I:(DE-He78)L301-20160331},
      pnm          = {312 - Functional and structural genomics (POF3-312)},
      pid          = {G:(DE-HGF)POF3-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29979988},
      doi          = {10.1016/j.stem.2018.06.003},
      url          = {https://inrepo02.dkfz.de/record/136836},
}