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@ARTICLE{Micakovic:137573,
author = {T. Micakovic and S. Papagiannarou and E. Clark and Y. Kuzay
and K. Abramovic and J. Peters and C. Sticht and N. Volk and
T. Fleming and P. Nawroth and H.-P. Hammes and N. Alenina
and H.-J. Gröne$^*$ and S. C. Hoffmann},
title = {{T}he angiotensin {II} type 2 receptors protect renal
tubule mitochondria in early stages of diabetes mellitus.},
journal = {Kidney international},
volume = {94},
number = {5},
issn = {0085-2538},
address = {Basingstoke},
publisher = {Nature Publishing Group},
reportid = {DKFZ-2018-01453},
pages = {937-950},
year = {2018},
abstract = {Diabetic nephropathy correlates more closely to defective
mitochondria and increased oxidative stress in the kidney
than to hyperglycemia. A key driving factor of diabetic
nephropathy is angiotensin II acting via the
G-protein-coupled cell membrane type 1 receptor. The present
study aimed to investigate the role of the angiotensin II
type 2 receptor (AT2R) at the early stages of diabetic
nephropathy. Using receptor binding studies and
immunohistochemistry we found that the mitochondria in renal
tubules contain high-affinity AT2Rs. Increased renal
mitochondrial AT2R density by transgenic overexpression was
associated with reduced superoxide production of isolated
mitochondria from non-diabetic rats. Streptozotocin-induced
diabetes (28 days) caused a drop in the ATP/oxygen ratio and
an increase in the superoxide production of isolated renal
mitochondria from wild-type diabetic rats. This correlated
with changes in the renal expression profile and increased
tubular epithelial cell proliferation. AT2R overexpression
in tubular epithelial cells inhibited all diabetes-induced
renal changes including a drop in mitochondrial
bioenergetics efficiency, a rise in mitochondrial superoxide
production, metabolic reprogramming, and increased
proliferation. Thus, AT2Rs translocate to mitochondria and
can contribute to reno-protective effects at early stages of
diabetes. Hence, targeted AT2R overexpression in renal cells
may open new avenues to develop novel types of drugs
preventing diabetic nephropathy.},
cin = {G130},
ddc = {610},
cid = {I:(DE-He78)G130-20160331},
pnm = {322 - Genetics and Pathophysiology (POF3-322)},
pid = {G:(DE-HGF)POF3-322},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30190172},
doi = {10.1016/j.kint.2018.06.006},
url = {https://inrepo02.dkfz.de/record/137573},
}