TY  - JOUR
AU  - Bochtler, Tilmann
AU  - Kartal-Kaess, Mutlu
AU  - Granzow, Martin
AU  - Hielscher, Thomas
AU  - Cosenza, Marco Raffaele
AU  - Herold-Mende, Christel
AU  - Jauch, Anna
AU  - Krämer, Alwin
TI  - Micronucleus formation in human cancer cells is biased by chromosome size.
JO  - Genes, chromosomes & cancer
VL  - 58
IS  - 6
SN  - 1045-2257
CY  - New York, NY
PB  - Wiley-Liss
M1  - DKFZ-2018-01921
SP  - 392-395
PY  - 2019
AB  - Chromosomal instability is one of the hallmarks of cancer and caused by chromosome missegregation during mitosis, a process frequently associated with micronucleus formation. Micronuclei are formed when chromosomes fail to join a daughter nucleus during cell division and are surrounded by their own nuclear membrane. Although it has been commonly assumed that the gain or loss of specific chromosomes is random during compromised cell division, recent data suggest that the size of chromosomes can impact on chromosome segregation fidelity. To test whether chromosome missegregation rates scale with chromosome size in primary human cancer cells, we assessed chromosome sequestration into micronuclei in patient-derived primary NCH149 glioblastoma cells, which display high-level numerical chromosome instability (CIN), pronounced spontaneous micronucleus formation but virtually no structural CIN. The cells were analysed by interphase fluorescence-in-situ-hybridization (FISH) using chromosome-specific painting probes for all chromosomes. Overall, 33
LB  - PUB:(DE-HGF)16
C6  - pmid:30411433
DO  - DOI:10.1002/gcc.22707
UR  - https://inrepo02.dkfz.de/record/141415
ER  -