TY  - JOUR
AU  - Burdyniuk, Mariia
AU  - Wesolowska, Natalia
AU  - Fleszar, Michal
AU  - Karreman, Matthia A
AU  - Machado, Pedro
AU  - Borrego-Pinto, Joana
AU  - Ruthensteiner, Bernhard
AU  - Schwab, Yannick
AU  - Lénárt, Péter
TI  - Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos.
JO  - Methods in cell biology
VL  - 145
SN  - 0091-679X
CY  - New York, NY [u.a.]
PB  - Elsevier
M1  - DKFZ-2018-02223
SP  - 293-313
PY  - 2018
AB  - The rapid and synchronous divisions of large and transparent oocytes, eggs, and embryos of marine species are exceptionally well suited for microscopic observation. Consequently, these cells have been models for cell division research since its beginnings and contributed some of its first and most fundamental discoveries. While large size and rapid transitions render these cells ideal specimens for light microscopy, the same features constitute a challenge for electron microscopy. Here, we describe example protocols from our work on starfish oocyte meiosis, where we overcome these challenges by using live imaging of fluorescently labeled structures in combination with correlated electron microscopy. In this work, we demonstrate how: (i) to capture a rapid, transient event in time and (ii) to localize a small structure within the large oocyte. These techniques are applicable with minor modifications to oocytes and embryos of other species and, possibly, to other cell types.
LB  - PUB:(DE-HGF)16
C6  - pmid:29957211
DO  - DOI:10.1016/bs.mcb.2018.03.031
UR  - https://inrepo02.dkfz.de/record/141993
ER  -