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@ARTICLE{Burdyniuk:141993,
author = {M. Burdyniuk and N. Wesolowska and M. Fleszar and M. A.
Karreman$^*$ and P. Machado and J. Borrego-Pinto and B.
Ruthensteiner and Y. Schwab and P. Lénárt},
title = {{C}orrelated light and electron microscopy of cell division
in large marine oocytes, eggs, and embryos.},
journal = {Methods in cell biology},
volume = {145},
issn = {0091-679X},
address = {New York, NY [u.a.]},
publisher = {Elsevier},
reportid = {DKFZ-2018-02223},
pages = {293-313},
year = {2018},
abstract = {The rapid and synchronous divisions of large and
transparent oocytes, eggs, and embryos of marine species are
exceptionally well suited for microscopic observation.
Consequently, these cells have been models for cell division
research since its beginnings and contributed some of its
first and most fundamental discoveries. While large size and
rapid transitions render these cells ideal specimens for
light microscopy, the same features constitute a challenge
for electron microscopy. Here, we describe example protocols
from our work on starfish oocyte meiosis, where we overcome
these challenges by using live imaging of fluorescently
labeled structures in combination with correlated electron
microscopy. In this work, we demonstrate how: (i) to capture
a rapid, transient event in time and (ii) to localize a
small structure within the large oocyte. These techniques
are applicable with minor modifications to oocytes and
embryos of other species and, possibly, to other cell
types.},
subtyp = {Review Article},
cin = {G370},
ddc = {570},
cid = {I:(DE-He78)G370-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29957211},
doi = {10.1016/bs.mcb.2018.03.031},
url = {https://inrepo02.dkfz.de/record/141993},
}