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000142202 1001_ $$0P:(DE-He78)2a8fbc2efe7e5e468472d57f724fe39b$$aZuckermann, Marc$$b0$$eFirst author
000142202 245__ $$aA novel cloning strategy for one-step assembly of multiplex CRISPR vectors.
000142202 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2018
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000142202 520__ $$aOne key advantage of the CRISPR/Cas9 system in comparison with other gene editing approaches lies in its potential for multiplexing. Here, we describe an elaborate procedure that allows the assembly of multiple gRNA expression cassettes into a vector of choice within a single step, termed ASAP(Adaptable System for Assembly of multiplexed Plasmids)-cloning. We demonstrate the utility of ASAP-cloning for multiple CRISPR-mediated applications, including efficient multiplex gene editing, robust transcription activation and convenient analysis of Cas9 activity in the presence of multiple gRNAs.
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000142202 7001_ $$0P:(DE-He78)aa1da4918a48503d125ee3325c7f135e$$aHlevnjak, Mario$$b1
000142202 7001_ $$0P:(DE-He78)a2b772a0a0db4a79b24771b8497dd9bd$$aYazdanparast, Haniyeh$$b2
000142202 7001_ $$0P:(DE-He78)1beba8f953e7ae7e96e8d3e9a48f10f7$$aZapatka, Marc$$b3
000142202 7001_ $$0P:(DE-He78)551bb92841f634070997aa168d818492$$aJones, David$$b4
000142202 7001_ $$0P:(DE-He78)e13b4363c5fe858044ef8a39c02c870c$$aLichter, Peter$$b5
000142202 7001_ $$0P:(DE-He78)bb186a8b38fef24ebd6f11918ade985d$$aGronych, Jan$$b6$$eLast author
000142202 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-018-35727-3$$gVol. 8, no. 1, p. 17499$$n1$$p17499$$tScientific reports$$v8$$x2045-2322$$y2018
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