TY  - JOUR
AU  - Zuckermann, Marc
AU  - Hlevnjak, Mario
AU  - Yazdanparast, Haniyeh
AU  - Zapatka, Marc
AU  - Jones, David
AU  - Lichter, Peter
AU  - Gronych, Jan
TI  - A novel cloning strategy for one-step assembly of multiplex CRISPR vectors.
JO  - Scientific reports
VL  - 8
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DKFZ-2019-00016
SP  - 17499
PY  - 2018
AB  - One key advantage of the CRISPR/Cas9 system in comparison with other gene editing approaches lies in its potential for multiplexing. Here, we describe an elaborate procedure that allows the assembly of multiple gRNA expression cassettes into a vector of choice within a single step, termed ASAP(Adaptable System for Assembly of multiplexed Plasmids)-cloning. We demonstrate the utility of ASAP-cloning for multiple CRISPR-mediated applications, including efficient multiplex gene editing, robust transcription activation and convenient analysis of Cas9 activity in the presence of multiple gRNAs.
LB  - PUB:(DE-HGF)16
C6  - pmid:30504793
C2  - pmc:PMC6269432
DO  - DOI:10.1038/s41598-018-35727-3
UR  - https://inrepo02.dkfz.de/record/142202
ER  -