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@ARTICLE{Zuckermann:142202,
      author       = {M. Zuckermann$^*$ and M. Hlevnjak$^*$ and H.
                      Yazdanparast$^*$ and M. Zapatka$^*$ and D. Jones$^*$ and P.
                      Lichter$^*$ and J. Gronych$^*$},
      title        = {{A} novel cloning strategy for one-step assembly of
                      multiplex {CRISPR} vectors.},
      journal      = {Scientific reports},
      volume       = {8},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DKFZ-2019-00016},
      pages        = {17499},
      year         = {2018},
      abstract     = {One key advantage of the CRISPR/Cas9 system in comparison
                      with other gene editing approaches lies in its potential for
                      multiplexing. Here, we describe an elaborate procedure that
                      allows the assembly of multiple gRNA expression cassettes
                      into a vector of choice within a single step, termed
                      ASAP(Adaptable System for Assembly of multiplexed
                      Plasmids)-cloning. We demonstrate the utility of
                      ASAP-cloning for multiple CRISPR-mediated applications,
                      including efficient multiplex gene editing, robust
                      transcription activation and convenient analysis of Cas9
                      activity in the presence of multiple gRNAs.},
      cin          = {B060 / B062 / L101},
      ddc          = {600},
      cid          = {I:(DE-He78)B060-20160331 / I:(DE-He78)B062-20160331 /
                      I:(DE-He78)L101-20160331},
      pnm          = {312 - Functional and structural genomics (POF3-312)},
      pid          = {G:(DE-HGF)POF3-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30504793},
      pmc          = {pmc:PMC6269432},
      doi          = {10.1038/s41598-018-35727-3},
      url          = {https://inrepo02.dkfz.de/record/142202},
}