% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@ARTICLE{Andersson:142742,
author = {E. I. Andersson and S. Pützer and B. Yadav and O. Dufva
and S. Khan and L. He and L. Sellner$^*$ and A. Schrader and
G. Crispatzu and M. Oleś and H. Zhang and S. Adnan-Awad and
S. Lagström and D. Bellanger and J. P. Mpindi and S.
Eldfors and T. Pemovska and P. Pietarinen and A. Lauhio and
K. Tomska$^*$ and C. Cuesta-Mateos and E. Faber and S.
Koschmieder and T. H. Brümmendorf and S. Kytölä and E.-R.
Savolainen and T. Siitonen and P. Ellonen and O. Kallioniemi
and K. Wennerberg and W. Ding and M.-H. Stern and W. Huber
and S. Anders and J. Tang and T. Aittokallio and T. Zenz$^*$
and M. Herling and S. Mustjoki},
title = {{D}iscovery of novel drug sensitivities in {T}-{PLL} by
high-throughput ex vivo drug testing and mutation
profiling.},
journal = {Leukemia},
volume = {32},
number = {3},
issn = {1476-5551},
address = {London},
publisher = {Springer Nature},
reportid = {DKFZ-2019-00459},
pages = {774 - 787},
year = {2018},
abstract = {T-cell prolymphocytic leukemia (T-PLL) is a rare and
aggressive neoplasm of mature T-cells with an urgent need
for rationally designed therapies to address its notoriously
chemo-refractory behavior. The median survival of T-PLL
patients is <2 years and clinical trials are difficult to
execute. Here we systematically explored the diversity of
drug responses in T-PLL patient samples using an ex vivo
drug sensitivity and resistance testing platform and
correlated the findings with somatic mutations and gene
expression profiles. Intriguingly, all T-PLL samples were
sensitive to the cyclin-dependent kinase inhibitor SNS-032,
which overcame stromal-cell-mediated protection and elicited
robust p53-activation and apoptosis. Across all patients,
the most effective classes of compounds were histone
deacetylase, phosphoinositide-3 kinase/AKT/mammalian target
of rapamycin, heat-shock protein 90 and BH3-family protein
inhibitors as well as p53 activators, indicating previously
unexplored, novel targeted approaches for treating T-PLL.
Although Janus-activated kinase-signal transducer and
activator of transcription factor (JAK-STAT) pathway
mutations were common in T-PLL $(71\%$ of patients),
JAK-STAT inhibitor responses were not directly linked to
those or other T-PLL-specific lesions. Overall, we found
that genetic markers do not readily translate into novel
effective therapeutic vulnerabilities. In conclusion, novel
classes of compounds with high efficacy in T-PLL were
discovered with the comprehensive ex vivo drug screening
platform warranting further studies of synergisms and
clinical testing.},
keywords = {Antineoplastic Agents (NLM Chemicals) / Biomarkers, Tumor
(NLM Chemicals) /
N-(5-(((5-(1,1-dimethylethyl)-2-oxazolyl)methyl)thio)-2-thiazolyl)-4-piperidinecarboxamide
(NLM Chemicals) / Oxazoles (NLM Chemicals) / Protein Kinase
Inhibitors (NLM Chemicals) / STAT Transcription Factors (NLM
Chemicals) / Thiazoles (NLM Chemicals) / Janus Kinases (NLM
Chemicals)},
cin = {G100 / G250},
ddc = {610},
cid = {I:(DE-He78)G100-20160331 / I:(DE-He78)G250-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28804127},
doi = {10.1038/leu.2017.252},
url = {https://inrepo02.dkfz.de/record/142742},
}