%0 Journal Article %A Veldwijk, Marlon R %A Seibold, Petra %A Botma, Akke %A Helmbold, Irmgard %A Sperk, Elena %A Giordano, Frank A %A Gürth, Nicole %A Kirchner, Anne %A Behrens, Sabine %A Wenz, Frederik %A Chang-Claude, Jenny %A Herskind, Carsten %T Association of CD4+ Radiation-Induced Lymphocyte Apoptosis with Fibrosis and Telangiectasia after Radiotherapy in 272 Breast Cancer Patients with </td><td width="150"> %T gt;10-Year Follow-up. %J Clinical cancer research %V 25 %N 2 %@ 1557-3265 %C Philadelphia, Pa. [u.a.] %I AACR %M DKFZ-2019-00508 %P 562 - 572 %D 2019 %X Radiation-induced lymphocyte apoptosis (RILA) has been suggested as a predictive assay for adverse late reactions after radiotherapy. Thus, low RILA values of T-lymphocyte subpopulations have been associated with increased risk for various endpoints at 2 to 3 years of follow-up. The purpose was to test if such associations persist for specific endpoints (subcutaneous fibrosis, telangiectasia) in breast cancer patients with at least 10 years of follow-up.Experimental Design: Two hundred and seventy-two female patients who had received breast-conserving therapy within the German ISE study were included (median follow-up: 11.6 years). Radiotherapy-induced side effects were scored according to the Late Effects in Normal Tissues-Subjective, Objective, Management, and Analytic (LENT-SOMA) classification system. RILA in the CD4+, CD8+, and natural killer (NK) subpopulations from peripheral blood was analyzed by flow cytometry. Multivariate predictive modeling was performed including relevant clinical risk factors.Low CD4+ RILA was associated with increased risk for both fibrosis (P = 0.011) and telangiectasia (P < 0.001). For fibrosis, the association was stronger outside the surgical area (Fibout; P = 0.004) than within (Fibin; P = 0.17). Predictive multivariate modeling including clinical risk factors yielded OR of 3.48 (95 %F PUB:(DE-HGF)16 %9 Journal Article %$ pmid:30327309 %R 10.1158/1078-0432.CCR-18-0777 %U https://inrepo02.dkfz.de/record/142878