TY - JOUR AU - Veldwijk, Marlon R AU - Seibold, Petra AU - Botma, Akke AU - Helmbold, Irmgard AU - Sperk, Elena AU - Giordano, Frank A AU - Gürth, Nicole AU - Kirchner, Anne AU - Behrens, Sabine AU - Wenz, Frederik AU - Chang-Claude, Jenny AU - Herskind, Carsten TI - Association of CD4+ Radiation-Induced Lymphocyte Apoptosis with Fibrosis and Telangiectasia after Radiotherapy in 272 Breast Cancer Patients with </td><td width="150"> TI - gt;10-Year Follow-up. JO - Clinical cancer research VL - 25 IS - 2 SN - 1557-3265 CY - Philadelphia, Pa. [u.a.] PB - AACR M1 - DKFZ-2019-00508 SP - 562 - 572 PY - 2019 AB - Radiation-induced lymphocyte apoptosis (RILA) has been suggested as a predictive assay for adverse late reactions after radiotherapy. Thus, low RILA values of T-lymphocyte subpopulations have been associated with increased risk for various endpoints at 2 to 3 years of follow-up. The purpose was to test if such associations persist for specific endpoints (subcutaneous fibrosis, telangiectasia) in breast cancer patients with at least 10 years of follow-up.Experimental Design: Two hundred and seventy-two female patients who had received breast-conserving therapy within the German ISE study were included (median follow-up: 11.6 years). Radiotherapy-induced side effects were scored according to the Late Effects in Normal Tissues-Subjective, Objective, Management, and Analytic (LENT-SOMA) classification system. RILA in the CD4+, CD8+, and natural killer (NK) subpopulations from peripheral blood was analyzed by flow cytometry. Multivariate predictive modeling was performed including relevant clinical risk factors.Low CD4+ RILA was associated with increased risk for both fibrosis (P = 0.011) and telangiectasia (P < 0.001). For fibrosis, the association was stronger outside the surgical area (Fibout; P = 0.004) than within (Fibin; P = 0.17). Predictive multivariate modeling including clinical risk factors yielded OR of 3.48 (95 LB - PUB:(DE-HGF)16 C6 - pmid:30327309 DO - DOI:10.1158/1078-0432.CCR-18-0777 UR - https://inrepo02.dkfz.de/record/142878 ER -