% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Robson:142919,
      author       = {J. P. Robson and M. Remke$^*$ and M. Kool$^*$ and E. Julian
                      and A. Korshunov$^*$ and S. Pfister$^*$ and G. W. Osborne
                      and M. D. Taylor and B. Wainwright and B. A. Reynolds},
      title        = {{I}dentification of {CD}24 as a marker of {P}atched1
                      deleted medulloblastoma-initiating neural progenitor
                      cells.l401},
      journal      = {PLOS ONE},
      volume       = {14},
      number       = {1},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {DKFZ-2019-00547},
      pages        = {e0210665 -},
      year         = {2019},
      abstract     = {High morbidity and mortality are common traits of malignant
                      tumours and identification of the cells responsible is a
                      focus of on-going research. Many studies are now reporting
                      the use of antibodies specific to Clusters of
                      Differentiation (CD) cell surface antigens to identify
                      tumour-initiating cell (TIC) populations in neural tumours.
                      Medulloblastoma is one of the most common malignant brain
                      tumours in children and despite a considerable amount of
                      research investigating this tumour, the identity of the
                      TICs, and the means by which such cells can be targeted
                      remain largely unknown. Current prognostication and
                      stratification of medulloblastoma using clinical factors,
                      histology and genetic profiling have classified this tumour
                      into four main subgroups: WNT, Sonic hedgehog (SHH), Group 3
                      and Group 4. Of these subgroups, SHH remains one of the most
                      studied tumour groups due to the ability to model
                      medulloblastoma formation through targeted deletion of the
                      Shh pathway inhibitor Patched1 (Ptch1). Here we sought to
                      utilise CD antibody expression to identify and isolate TIC
                      populations in Ptch1 deleted medulloblastoma, and determine
                      if these antibodies can help classify the identity of human
                      medulloblastoma subgroups. Using a fluorescence-activated
                      cell sorted (FACS) CD antibody panel, we identified CD24 as
                      a marker of TICs in Ptch1 deleted medulloblastoma. CD24
                      expression was not correlated with markers of astrocytes or
                      oligodendrocytes, but co-labelled with markers of neural
                      progenitor cells. In conjunction with CD15, proliferating
                      CD24+/CD15+ granule cell precursors (GCPs) were identified
                      as a TIC population in Ptch1 deleted medulloblastoma. On
                      human medulloblastoma, CD24 was found to be highly expressed
                      on Group 3, Group 4 and SHH subgroups compared with the WNT
                      subgroup, which was predominantly positive for CD15,
                      suggesting CD24 is an important marker of non-WNT
                      medulloblastoma initiating cells and a potential therapeutic
                      target in human medulloblastoma. This study reports the use
                      of CD24 and CD15 to isolate a GCP-like TIC population in
                      Ptch1 deleted medulloblastoma, and suggests CD24 expression
                      as a marker to help stratify human WNT tumours from other
                      medulloblastoma subgroups.},
      cin          = {L401 / B062 / B300},
      ddc          = {610},
      cid          = {I:(DE-He78)L401-20160331 / I:(DE-He78)B062-20160331 /
                      I:(DE-He78)B300-20160331},
      pnm          = {312 - Functional and structural genomics (POF3-312)},
      pid          = {G:(DE-HGF)POF3-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30657775},
      pmc          = {pmc:PMC6338368},
      doi          = {10.1371/journal.pone.0210665},
      url          = {https://inrepo02.dkfz.de/record/142919},
}