%0 Journal Article
%A Jaunmuktane, Zane
%A Capper, David
%A Jones, David
%A Schrimpf, Daniel
%A Sill, Martin
%A Dutt, Monika
%A Suraweera, Nirosha
%A Pfister, Stefan
%A von Deimling, Andreas
%A Brandner, Sebastian
%T Methylation array profiling of adult brain tumours: diagnostic outcomes in a large, single centre.
%J Acta Neuropathologica Communications
%V 7
%N 1
%@ 2051-5960
%C London
%I Biomed Central
%M DKFZ-2019-00662
%P 24
%D 2019
%X The introduction of the classification of brain tumours based on their DNA methylation profile has significantly changed the diagnostic approach for cases with ambiguous histology, non-informative or contradictory molecular profiles or for entities where methylation profiling provides useful information for patient risk stratification, for example in medulloblastoma and ependymoma. We present our experience that combines a conventional molecular diagnostic approach with the complementary use of a DNA methylation-based classification tool, for adult brain tumours originating from local as well as national referrals. We report the frequency of IDH mutations in a large cohort of nearly 1550 patients, EGFR amplifications in almost 1900 IDH-wildtype glioblastomas, and histone mutations in 70 adult gliomas. We demonstrate how additional methylation-based classification has changed and improved our diagnostic approach. Of the 325 cases referred for methylome testing, 179 (56
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:30786920
%2 pmc:PMC6381711
%R 10.1186/s40478-019-0668-8
%U https://inrepo02.dkfz.de/record/143043