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@ARTICLE{Schbel:143372,
      author       = {R. Schübel$^*$ and D. Sookthai$^*$ and J. Greimel$^*$ and
                      T. S. Johnson$^*$ and M. E. Grafetstätter$^*$ and R.
                      Kirsten and M. Kratz and C. M. Ulrich and R. Kaaks$^*$ and
                      T. Kühn$^*$},
      title        = {{K}ey {G}enes of {L}ipid {M}etabolism and {WNT}-{S}ignaling
                      {A}re {D}ownregulated in {S}ubcutaneous {A}dipose {T}issue
                      with {M}oderate {W}eight {L}oss.},
      journal      = {Nutrients},
      volume       = {11},
      number       = {3},
      issn         = {2072-6643},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2019-00960},
      pages        = {639},
      year         = {2019},
      abstract     = {Smaller cross-sectional studies and bariatric surgery
                      trials suggest that weight loss may change the expression of
                      genes in adipose tissue that have been implicated in the
                      development of metabolic diseases, but well-powered
                      intervention trials are lacking. In post hoc analyses of
                      data from a 12-week dietary intervention trial initially
                      designed to compare metabolic effects of intermittent vs.
                      continuous calorie restriction, we analyzed the effects of
                      overall weight loss on the subcutaneous adipose tissue (SAT)
                      transcriptome. Changes in the transcriptome were measured by
                      microarray using SAT samples of 138 overweight or obese
                      individuals (age range: 35⁻65 years, BMI range: 25⁻40,
                      non-smokers, non-diabetics). Participants were grouped post
                      hoc according to the degree of their weight loss by
                      quartiles (average weight loss in quartiles 1 to 4: $0\%,$
                      $-3.2\%,$ $-5.9\%,$ and $-10.7\%).$ Candidate genes showing
                      differential expression with weight loss according to
                      microarray analyses were validated by reverse transcription
                      quantitative polymerase chain reaction (RT-qPCR), and fold
                      changes (FCs) were calculated to quantify differences in
                      gene expression. A comparison of individuals in the highest
                      vs. the lowest weight loss quartile revealed 681 genes to be
                      differentially expressed (corrected p < 0.05), with 40
                      showing FCs of at least 0.4. Out of these, expression
                      changes in secreted frizzled-related protein 2 (SFRP2, FC =
                      0.65, p = 0.006), stearoyl-CoA desaturase (SCD, FC = -1.00,
                      p < 0.001), and hypoxia inducible lipid droplet-associated
                      (HILPDA, FC = -0.45, p = 0.001) with weight loss were
                      confirmed by RT-qPCR. Dietary weight loss induces
                      significant changes in the expression of genes implicated in
                      lipid metabolism (SCD and HILPDA) and WNT-signaling (SFRP2)
                      in SAT.},
      cin          = {C020},
      ddc          = {610},
      cid          = {I:(DE-He78)C020-20160331},
      pnm          = {313 - Cancer risk factors and prevention (POF3-313)},
      pid          = {G:(DE-HGF)POF3-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30884788},
      doi          = {10.3390/nu11030639},
      url          = {https://inrepo02.dkfz.de/record/143372},
}