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@ARTICLE{Schbel:143372,
author = {R. Schübel$^*$ and D. Sookthai$^*$ and J. Greimel$^*$ and
T. S. Johnson$^*$ and M. E. Grafetstätter$^*$ and R.
Kirsten and M. Kratz and C. M. Ulrich and R. Kaaks$^*$ and
T. Kühn$^*$},
title = {{K}ey {G}enes of {L}ipid {M}etabolism and {WNT}-{S}ignaling
{A}re {D}ownregulated in {S}ubcutaneous {A}dipose {T}issue
with {M}oderate {W}eight {L}oss.},
journal = {Nutrients},
volume = {11},
number = {3},
issn = {2072-6643},
address = {Basel},
publisher = {MDPI},
reportid = {DKFZ-2019-00960},
pages = {639},
year = {2019},
abstract = {Smaller cross-sectional studies and bariatric surgery
trials suggest that weight loss may change the expression of
genes in adipose tissue that have been implicated in the
development of metabolic diseases, but well-powered
intervention trials are lacking. In post hoc analyses of
data from a 12-week dietary intervention trial initially
designed to compare metabolic effects of intermittent vs.
continuous calorie restriction, we analyzed the effects of
overall weight loss on the subcutaneous adipose tissue (SAT)
transcriptome. Changes in the transcriptome were measured by
microarray using SAT samples of 138 overweight or obese
individuals (age range: 35⁻65 years, BMI range: 25⁻40,
non-smokers, non-diabetics). Participants were grouped post
hoc according to the degree of their weight loss by
quartiles (average weight loss in quartiles 1 to 4: $0\%,$
$-3.2\%,$ $-5.9\%,$ and $-10.7\%).$ Candidate genes showing
differential expression with weight loss according to
microarray analyses were validated by reverse transcription
quantitative polymerase chain reaction (RT-qPCR), and fold
changes (FCs) were calculated to quantify differences in
gene expression. A comparison of individuals in the highest
vs. the lowest weight loss quartile revealed 681 genes to be
differentially expressed (corrected p < 0.05), with 40
showing FCs of at least 0.4. Out of these, expression
changes in secreted frizzled-related protein 2 (SFRP2, FC =
0.65, p = 0.006), stearoyl-CoA desaturase (SCD, FC = -1.00,
p < 0.001), and hypoxia inducible lipid droplet-associated
(HILPDA, FC = -0.45, p = 0.001) with weight loss were
confirmed by RT-qPCR. Dietary weight loss induces
significant changes in the expression of genes implicated in
lipid metabolism (SCD and HILPDA) and WNT-signaling (SFRP2)
in SAT.},
cin = {C020},
ddc = {610},
cid = {I:(DE-He78)C020-20160331},
pnm = {313 - Cancer risk factors and prevention (POF3-313)},
pid = {G:(DE-HGF)POF3-313},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30884788},
doi = {10.3390/nu11030639},
url = {https://inrepo02.dkfz.de/record/143372},
}