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@ARTICLE{Muenchau:143645,
      author       = {S. Muenchau and R. Deutsch and I. J. de Castro and T.
                      Hielscher$^*$ and N. Heber and B. Niesler and M. Lusic and
                      M. L. Stanifer and S. Boulant$^*$},
      title        = {{H}ypoxic environment promotes barrier formation in human
                      intestinal epithelial cells through regulation of
                      mi{RNA}-320a expression.},
      journal      = {Molecular and cellular biology},
      volume       = {39},
      number       = {14},
      issn         = {1098-5549},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {DKFZ-2019-01219},
      pages        = {e00553-18},
      year         = {2019},
      abstract     = {Intestinal epithelial cells (IECs) are exposed to the
                      low-oxygen environment present in the lumen of the gut.
                      These hypoxic conditions are on one hand fundamental for the
                      survival of the commensal microbiota, and on the other hand,
                      favor the formation of a selective semipermeable barrier
                      allowing IECs to transport essential nutrients/water while
                      keeping the sterile internal compartments separated from the
                      lumen containing commensals. The hypoxia-inducible factor
                      (HIF) complex, which allows cells to respond and adapt to
                      fluctuations in oxygen levels, has been described as a key
                      regulator in maintaining IEC barrier function by regulating
                      their tight junction integrity. In this study, we sought to
                      better evaluate the mechanisms by which low oxygen
                      conditions impact the barrier function of human IECs. By
                      profiling miRNA expression in IECs under hypoxia, we
                      identified miRNA-320a as a novel barrier formation
                      regulator. Using pharmacological inhibitors and short
                      hairpin RNA-mediated silencing we could demonstrate that
                      expression of this miRNA was HIF-dependent. Importantly,
                      using over-expression and knock-down approaches of
                      miRNA-320a we could confirm its direct role in the
                      regulation of barrier functions in human IECs. These results
                      reveal an important link between miRNA expression and
                      barrier integrity, providing a novel insight into mechanisms
                      of hypoxia-driven epithelial homeostasis.},
      cin          = {C060 / F140},
      ddc          = {570},
      cid          = {I:(DE-He78)C060-20160331 / I:(DE-He78)F140-20160331},
      pnm          = {316 - Infections and cancer (POF3-316)},
      pid          = {G:(DE-HGF)POF3-316},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31061092},
      doi          = {10.1128/MCB.00553-18},
      url          = {https://inrepo02.dkfz.de/record/143645},
}