TY - JOUR
AU - Wu, Jingxia
AU - Ma, Sicong
AU - Sandhoff, Roger
AU - Ming, Yanan
AU - Hotz-Wagenblatt, Agnes
AU - Timmerman, Vincent
AU - Bonello-Palot, Nathalie
AU - Schlotter-Weigel, Beate
AU - Auer-Grumbach, Michaela
AU - Seeman, Pavel
AU - Löscher, Wolfgang N
AU - Reindl, Markus
AU - Weiss, Florian
AU - Mah, Eric
AU - Weisshaar, Nina
AU - Madi, Alaa
AU - Mohr, Kerstin
AU - Schlimbach, Tilo
AU - Velasco Cárdenas, Rubí M-H
AU - Koeppel, Jonas
AU - Grünschläger, Florian
AU - Müller, Lisann
AU - Baumeister, Maren
AU - Brügger, Britta
AU - Schmitt, Michael
AU - Wabnitz, Guido
AU - Samstag, Yvonne
AU - Cui, Guoliang
TI - Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8+ T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness.
JO - Immunity
VL - 50
IS - 5
SN - 1074-7613
CY - New York, NY
PB - Elsevier
M1 - DKFZ-2019-01371
SP - 1218 - 1231.e5
PY - 2019
AB - Patients with the neurological disorder HSAN-I suffer frequent infections, attributed to a lack of pain sensation and failure to seek care for minor injuries. Whether protective CD8+ T cells are affected in HSAN-I patients remains unknown. Here, we report that HSAN-I-associated mutations in serine palmitoyltransferase subunit SPTLC2 dampened human T cell responses. Antigen stimulation and inflammation induced SPTLC2 expression, and murine T-cell-specific ablation of Sptlc2 impaired antiviral-T-cell expansion and effector function. Sptlc2 deficiency reduced sphingolipid biosynthetic flux and led to prolonged activation of the mechanistic target of rapamycin complex 1 (mTORC1), endoplasmic reticulum (ER) stress, and CD8+ T cell death. Protective CD8+ T cell responses in HSAN-I patient PBMCs and Sptlc2-deficient mice were restored by supplementing with sphingolipids and pharmacologically inhibiting ER stress-induced cell death. Therefore, SPTLC2 underpins protective immunity by translating extracellular stimuli into intracellular anabolic signals and antagonizes ER stress to promote T cell metabolic fitness.
LB - PUB:(DE-HGF)16
C6 - pmid:30952607
DO - DOI:10.1016/j.immuni.2019.03.005
UR - https://inrepo02.dkfz.de/record/143809
ER -