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000143883 0247_ $$2doi$$a10.3390/ijms20030704
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000143883 041__ $$aeng
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000143883 1001_ $$aWard Gahlawat, Aoife$$b0
000143883 245__ $$aEvaluation of Storage Tubes for Combined Analysis of Circulating Nucleic Acids in Liquid Biopsies.
000143883 260__ $$aBasel$$bMolecular Diversity Preservation International$$c2019
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000143883 520__ $$aIn the last decade, circulating nucleic acids such as microRNAs (miRNAs) and cell-free DNA (cfDNA) have become increasingly important in serving as potential novel biomarkers for a variety of human diseases. If cell-free nucleic acids are to become routinely used in diagnostics, the difference in plasma miRNA and cfDNA levels between healthy and diseased subjects must exceed pre-analytical and analytical variability. Until now, few studies have addressed the time limitations of pre-processing or explored the potential use of long-term blood storage tubes, which might need to be implemented in real-life diagnostics. In this study, we analyzed the stability of four breast cancer-associated miRNAs and two cancer-associated genes under various storage conditions, to test their limitations for potential application in clinical diagnostics. In two consecutive experiments, we tested the limits of conventional EDTA tubes, as well as long-term storage blood collection tubes (BCTs) from four different manufacturers. We found that circulating miRNAs are relatively stable when stored in EDTA monovettes for up to 12 h before processing. When stored in BCTs, circulating miRNAs and cfDNA are stable for up to 7 days, depending on the manufacturer. Norgen tubes were superior for cfDNA yield, while Streck tubes performed the worst in our study with hemolysis induction. In conclusion, plasma prepared from whole blood is suitable for the quantification of both cf-miRNAs and cfDNA simultaneously.
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000143883 650_7 $$2NLM Chemicals$$aBiomarkers
000143883 650_7 $$2NLM Chemicals$$aCell-Free Nucleic Acids
000143883 650_7 $$2NLM Chemicals$$aCirculating MicroRNA
000143883 7001_ $$aLenhardt, Judith$$b1
000143883 7001_ $$aWitte, Tania$$b2
000143883 7001_ $$aKeitel, Denise$$b3
000143883 7001_ $$0P:(DE-He78)48a0f04bbec9855553033c79ea75ce94$$aKaufhold, Anna$$b4$$udkfz
000143883 7001_ $$0P:(DE-He78)5100059e746b377e2e0a37c0e24f6bf7$$aMaass, Kendra K$$b5$$udkfz
000143883 7001_ $$0P:(DE-He78)a7c1bbac024fa232d9c6b78443328d9d$$aPajtler, Kristian W$$b6$$udkfz
000143883 7001_ $$aSohn, Christof$$b7
000143883 7001_ $$aSchott, Sarah$$b8
000143883 773__ $$0PERI:(DE-600)2019364-6$$a10.3390/ijms20030704$$gVol. 20, no. 3, p. 704 -$$n3$$p704 -$$tInternational journal of molecular sciences$$v20$$x1422-0067$$y2019
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