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@ARTICLE{WardGahlawat:143883,
      author       = {A. Ward Gahlawat and J. Lenhardt and T. Witte and D. Keitel
                      and A. Kaufhold$^*$ and K. K. Maass$^*$ and K. W.
                      Pajtler$^*$ and C. Sohn and S. Schott},
      title        = {{E}valuation of {S}torage {T}ubes for {C}ombined {A}nalysis
                      of {C}irculating {N}ucleic {A}cids in {L}iquid {B}iopsies.},
      journal      = {International journal of molecular sciences},
      volume       = {20},
      number       = {3},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {DKFZ-2019-01445},
      pages        = {704 -},
      year         = {2019},
      abstract     = {In the last decade, circulating nucleic acids such as
                      microRNAs (miRNAs) and cell-free DNA (cfDNA) have become
                      increasingly important in serving as potential novel
                      biomarkers for a variety of human diseases. If cell-free
                      nucleic acids are to become routinely used in diagnostics,
                      the difference in plasma miRNA and cfDNA levels between
                      healthy and diseased subjects must exceed pre-analytical and
                      analytical variability. Until now, few studies have
                      addressed the time limitations of pre-processing or explored
                      the potential use of long-term blood storage tubes, which
                      might need to be implemented in real-life diagnostics. In
                      this study, we analyzed the stability of four breast
                      cancer-associated miRNAs and two cancer-associated genes
                      under various storage conditions, to test their limitations
                      for potential application in clinical diagnostics. In two
                      consecutive experiments, we tested the limits of
                      conventional EDTA tubes, as well as long-term storage blood
                      collection tubes (BCTs) from four different manufacturers.
                      We found that circulating miRNAs are relatively stable when
                      stored in EDTA monovettes for up to 12 h before processing.
                      When stored in BCTs, circulating miRNAs and cfDNA are stable
                      for up to 7 days, depending on the manufacturer. Norgen
                      tubes were superior for cfDNA yield, while Streck tubes
                      performed the worst in our study with hemolysis induction.
                      In conclusion, plasma prepared from whole blood is suitable
                      for the quantification of both cf-miRNAs and cfDNA
                      simultaneously.},
      keywords     = {Biomarkers (NLM Chemicals) / Cell-Free Nucleic Acids (NLM
                      Chemicals) / Circulating MicroRNA (NLM Chemicals)},
      cin          = {B062},
      ddc          = {540},
      cid          = {I:(DE-He78)B062-20160331},
      pnm          = {312 - Functional and structural genomics (POF3-312)},
      pid          = {G:(DE-HGF)POF3-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30736351},
      pmc          = {pmc:PMC6387045},
      doi          = {10.3390/ijms20030704},
      url          = {https://inrepo02.dkfz.de/record/143883},
}