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@ARTICLE{Nies:143934,
      author       = {C. Nies and T. Rubner$^*$ and H. Lorig and V. Colditz and
                      H. Seelmann and A. Müller and E. Gottwald},
      title        = {{A} {M}icrocavity {A}rray-{B}ased 4{D} {C}ell {C}ulture
                      {P}latform.},
      journal      = {Bioengineering},
      volume       = {6},
      number       = {2},
      issn         = {2306-5354},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2019-01491},
      pages        = {50},
      year         = {2019},
      abstract     = {(1) Background: We describe a 4D cell culture platform with
                      which we tried to detect and to characterize migration
                      dynamics of single hematopoietic stem cells in polymer film
                      microcavity arrays integrated into a microtiter plate. (2)
                      Methods: The system was set up with CD34-expressing KG-1a
                      cells as a surrogate for hematopoietic stem cells. We then
                      evaluated the system as an artificial hematopoietic stem
                      cell niche model comprised of a co-culture of human
                      hematopoietic stem cells from cord blood (cord blood CD34+
                      cells, hHSCs) and human mesenchymal stromal cells (hMSCs)
                      from bone marrow over a period of 21 days. We used a
                      software-based cell detection method to count single
                      hematopoietic stem cells (HSCs) in microcavities. (3)
                      Results: It was possible to detect single HSCs and their
                      migration behavior within single microcavities. The HSCs
                      displayed a pronounced migration behavior with one
                      population of CD34-expressing cells located at the bottom of
                      the microcavities and one population located in the middle
                      of the microcavities at day 14. However, at day 21 the two
                      populations seemed to unite again so that no clear
                      distinction between the two was possible anymore. (4)
                      Conclusions: Single cell migration detection was possible
                      but microscopy and flow cytometry delivered non-uniform data
                      sets. Further optimization is currently being developed.},
      cin          = {W220},
      ddc          = {570},
      cid          = {I:(DE-He78)W220-20160331},
      pnm          = {319H - Addenda (POF3-319H)},
      pid          = {G:(DE-HGF)POF3-319H},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31159244},
      doi          = {10.3390/bioengineering6020050},
      url          = {https://inrepo02.dkfz.de/record/143934},
}