000144166 001__ 144166
000144166 005__ 20240229112619.0
000144166 0247_ $$2doi$$a10.1038/s41598-019-45237-5
000144166 0247_ $$2pmid$$apmid:31222112
000144166 0247_ $$2pmc$$apmc:PMC6586633
000144166 0247_ $$2altmetric$$aaltmetric:62550897
000144166 037__ $$aDKFZ-2019-01715
000144166 041__ $$aeng
000144166 082__ $$a600
000144166 1001_ $$aNguyen, Chi D L$$b0
000144166 245__ $$aA sensitive and simple targeted proteomics approach to quantify transcription factor and membrane proteins of the unfolded protein response pathway in glioblastoma cells.
000144166 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2019
000144166 3367_ $$2DRIVER$$aarticle
000144166 3367_ $$2DataCite$$aOutput Types/Journal article
000144166 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1562230164_4521
000144166 3367_ $$2BibTeX$$aARTICLE
000144166 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000144166 3367_ $$00$$2EndNote$$aJournal Article
000144166 520__ $$aMany cellular events are driven by changes in protein expression, measurable by mass spectrometry or antibody-based assays. However, using conventional technology, the analysis of transcription factor or membrane receptor expression is often limited by an insufficient sensitivity and specificity. To overcome this limitation, we have developed a high-resolution targeted proteomics strategy, which allows quantification down to the lower attomol range in a straightforward way without any prior enrichment or fractionation approaches. The method applies isotope-labeled peptide standards for quantification of the protein of interest. As proof of principle, we applied the improved workflow to proteins of the unfolded protein response (UPR), a signaling pathway of great clinical importance, and could for the first time detect and quantify all major UPR receptors, transducers and effectors that are not readily detectable via antibody-based-, SRM- or conventional PRM assays. As transcription and translation is central to the regulation of UPR, quantification and determination of protein copy numbers in the cell is important for our understanding of the signaling process as well as how pharmacologic modulation of these pathways impacts on the signaling. These questions can be answered using our newly established workflow as exemplified in an experiment using UPR perturbation in a glioblastoma cell lines.
000144166 536__ $$0G:(DE-HGF)POF3-319H$$a319H - Addenda (POF3-319H)$$cPOF3-319H$$fPOF III$$x0
000144166 588__ $$aDataset connected to CrossRef, PubMed,
000144166 7001_ $$aMalchow, Sebastian$$b1
000144166 7001_ $$aReich, Stefan$$b2
000144166 7001_ $$aSteltgens, Sascha$$b3
000144166 7001_ $$aShuvaev, Konstantin V$$b4
000144166 7001_ $$aLoroch, Stefan$$b5
000144166 7001_ $$aLorenz, Christin$$b6
000144166 7001_ $$aSickmann, Albert$$b7
000144166 7001_ $$00000-0003-1231-0076$$aKnobbe-Thomsen, Christiane B$$b8
000144166 7001_ $$0P:(DE-He78)a33ae52a1d80b847405db3ab83b9e90d$$aTews, Björn$$b9$$udkfz
000144166 7001_ $$aMedenbach, Jan$$b10
000144166 7001_ $$00000-0003-0232-3375$$aAhrends, Robert$$b11
000144166 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-019-45237-5$$gVol. 9, no. 1, p. 8836$$n1$$p8836$$tScientific reports$$v9$$x2045-2322$$y2019
000144166 909CO $$ooai:inrepo02.dkfz.de:144166$$pVDB
000144166 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)a33ae52a1d80b847405db3ab83b9e90d$$aDeutsches Krebsforschungszentrum$$b9$$kDKFZ
000144166 9131_ $$0G:(DE-HGF)POF3-319H$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vAddenda$$x0
000144166 9141_ $$y2019
000144166 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bSCI REP-UK : 2017
000144166 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000144166 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000144166 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000144166 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central
000144166 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000144166 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000144166 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review
000144166 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ
000144166 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000144166 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000144166 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000144166 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000144166 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000144166 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000144166 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000144166 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record
000144166 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000144166 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000144166 9201_ $$0I:(DE-He78)V077-20160331$$kV077$$lAG Molekulare Mechanismen der Tumorzell-Invasion$$x0
000144166 980__ $$ajournal
000144166 980__ $$aVDB
000144166 980__ $$aI:(DE-He78)V077-20160331
000144166 980__ $$aUNRESTRICTED