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@ARTICLE{Nguyen:144166,
      author       = {C. D. L. Nguyen and S. Malchow and S. Reich and S.
                      Steltgens and K. V. Shuvaev and S. Loroch and C. Lorenz and
                      A. Sickmann and C. B. Knobbe-Thomsen and B. Tews$^*$ and J.
                      Medenbach and R. Ahrends},
      title        = {{A} sensitive and simple targeted proteomics approach to
                      quantify transcription factor and membrane proteins of the
                      unfolded protein response pathway in glioblastoma cells.},
      journal      = {Scientific reports},
      volume       = {9},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DKFZ-2019-01715},
      pages        = {8836},
      year         = {2019},
      abstract     = {Many cellular events are driven by changes in protein
                      expression, measurable by mass spectrometry or
                      antibody-based assays. However, using conventional
                      technology, the analysis of transcription factor or membrane
                      receptor expression is often limited by an insufficient
                      sensitivity and specificity. To overcome this limitation, we
                      have developed a high-resolution targeted proteomics
                      strategy, which allows quantification down to the lower
                      attomol range in a straightforward way without any prior
                      enrichment or fractionation approaches. The method applies
                      isotope-labeled peptide standards for quantification of the
                      protein of interest. As proof of principle, we applied the
                      improved workflow to proteins of the unfolded protein
                      response (UPR), a signaling pathway of great clinical
                      importance, and could for the first time detect and quantify
                      all major UPR receptors, transducers and effectors that are
                      not readily detectable via antibody-based-, SRM- or
                      conventional PRM assays. As transcription and translation is
                      central to the regulation of UPR, quantification and
                      determination of protein copy numbers in the cell is
                      important for our understanding of the signaling process as
                      well as how pharmacologic modulation of these pathways
                      impacts on the signaling. These questions can be answered
                      using our newly established workflow as exemplified in an
                      experiment using UPR perturbation in a glioblastoma cell
                      lines.},
      cin          = {V077},
      ddc          = {600},
      cid          = {I:(DE-He78)V077-20160331},
      pnm          = {319H - Addenda (POF3-319H)},
      pid          = {G:(DE-HGF)POF3-319H},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31222112},
      pmc          = {pmc:PMC6586633},
      doi          = {10.1038/s41598-019-45237-5},
      url          = {https://inrepo02.dkfz.de/record/144166},
}