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@ARTICLE{Oswald:144397,
author = {E. Oswald$^*$ and M. Kirschberg and F. Aubin and A.
Alonso$^*$ and M. Hufbauer and B. Akgül and E. Auvinen},
title = {{B}eta{HPV} {E}6 and {E}7 colocalize with {N}u{M}a in
dividing keratinocytes.},
journal = {Virus genes},
volume = {55},
number = {5},
issn = {1572-994X},
address = {Dordrecht [u.a.]},
publisher = {Springer Science + Business Media B.V},
reportid = {DKFZ-2019-01850},
pages = {600-609},
year = {2019},
abstract = {Human papillomaviruses (HPVs) of genus betapapillomavirus
(betaHPV) are implicated in skin carcinogenesis, but their
exact role in keratinocyte transformation is poorly
understood. We show an interaction of HPV5 and HPV8
oncoproteins E6 and E7 with the nuclear mitotic apparatus
protein 1 (NuMA). Binding of E6 or E7 to NuMA induces little
aneuploidy, cell cycle alterations, or aberrant centrosomes.
Intracellular localization of NuMA is not altered by E6 and
E7 expression in 2D cultures. However, the localization
profile is predominantly cytoplasmic in 3D organotypic skin
models. Both viral proteins colocalize with NuMA in
interphase cells, while only E7 colocalizes with NuMA in
mitotic cells. Intriguingly, a small subset of cells shows
E7 at only one spindle pole, whereas NuMA is present at both
poles. This dissimilar distribution of E7 at the spindle
poles may alter cell differentiation, which may in turn be
relevant for betaHPV-induced skin carcinogenesis.},
cin = {F050},
ddc = {610},
cid = {I:(DE-He78)F050-20160331},
pnm = {316 - Infections and cancer (POF3-316)},
pid = {G:(DE-HGF)POF3-316},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31290065},
doi = {10.1007/s11262-019-01685-9},
url = {https://inrepo02.dkfz.de/record/144397},
}