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@ARTICLE{Tomska:144469,
author = {K. Tomska$^*$ and S. Scheinost$^*$ and T. Zenz},
title = {{L}ymphoma and {L}eukemia {C}ell {V}ulnerabilities and
{R}esistance {I}dentified by {C}ompound {L}ibrary
{S}creens.},
journal = {Methods in molecular biology},
volume = {1956},
issn = {1064-3745},
address = {[Heidelberg]},
publisher = {[Springer]},
reportid = {DKFZ-2019-01920},
isbn = {978-1-4939-9150-1 (print)},
pages = {351-362},
year = {2019},
abstract = {Response to anticancer agents is often restricted to
subsets of patients. The recognition of factors underlying
this heterogeneity and the identification of biomarkers
associated with response to drugs would greatly improve the
efficacy of drug treatment. Platforms that can
comprehensively map drug response in high-throughput ex vivo
provide a unique tool to identify associated biomarkers and
provide hypotheses for mechanisms underlying variable
response. Such screens can be performed on cell lines and
short-term cultures of primary cells to take advantage of
the respective models' strength, which include, e.g., the
ability to silence genes in cell lines and the 'indefinite'
supply of primary cells where clonal selection can be
avoided. Cohorts of such samples represent the natural
diversity of cancers, including rarer mutations and
combinatorial patterns of mutations.We here summarize a
simple and scalable method for the measurement of viability
after drug exposure based on ATP measurements as a surrogate
for viability, which we use to measure and understand drug
response in cell lines and primary cells.},
keywords = {Antineoplastic Agents (NLM Chemicals) / Biomarkers,
Pharmacological (NLM Chemicals)},
cin = {G250},
ddc = {570},
cid = {I:(DE-He78)G250-20160331},
pnm = {319H - Addenda (POF3-319H)},
pid = {G:(DE-HGF)POF3-319H},
typ = {PUB:(DE-HGF)3 / PUB:(DE-HGF)16},
pubmed = {pmid:30779044},
doi = {10.1007/978-1-4939-9151-8_17},
url = {https://inrepo02.dkfz.de/record/144469},
}