TY  - JOUR
AU  - Doroudgar, Shirin
AU  - Hofmann, Christoph
AU  - Boileau, Etienne
AU  - Malone, Brandon
AU  - Riechert, Eva
AU  - Gorska, Agnieszka A
AU  - Jakobi, Tobias
AU  - Sandmann, Clara
AU  - Jürgensen, Lonny
AU  - Kmietczyk, Vivien
AU  - Malovrh, Ellen
AU  - Burghaus, Jana
AU  - Rettel, Mandy
AU  - Stein, Frank
AU  - Younesi, Fereshteh
AU  - Friedrich, Ulrike A
AU  - Mauz, Victoria
AU  - Backs, Johannes
AU  - Kramer, Günter
AU  - Katus, Hugo A
AU  - Dieterich, Christoph
AU  - Völkers, Mirko
TI  - Monitoring Cell-Type-Specific Gene Expression Using Ribosome Profiling In Vivo During Cardiac Hemodynamic Stress.
JO  - Circulation research
VL  - 125
IS  - 4
SN  - 1524-4571
CY  - New York, NY
PB  - Assoc.
M1  - DKFZ-2019-01978
SP  - 431 - 448
PY  - 2019
N1  - DKFZ-ZMBH Alliance
AB  - Gene expression profiles have been mainly determined by analysis of transcript abundance. However, these analyses cannot capture posttranscriptional gene expression control at the level of translation, which is a key step in the regulation of gene expression, as evidenced by the fact that transcript levels often poorly correlate with protein levels. Furthermore, genome-wide transcript profiling of distinct cell types is challenging due to the fact that lysates from tissues always represent a mixture of cells.This study aimed to develop a new experimental method that overcomes both limitations and to apply this method to perform a genome-wide analysis of gene expression on the translational level in response to pressure overload.By combining ribosome profiling (Ribo-seq) with a ribosome-tagging approach (Ribo-tag), it was possible to determine the translated transcriptome in specific cell types from the heart. After pressure overload, we monitored the cardiac myocyte translatome by purifying tagged cardiac myocyte ribosomes from cardiac lysates and subjecting the ribosome-protected mRNA fragments to deep sequencing. We identified subsets of mRNAs that are regulated at the translational level and found that translational control determines early changes in gene expression in response to cardiac stress in cardiac myocytes. Translationally controlled transcripts are associated with specific biological processes related to translation, protein quality control, and metabolism. Mechanistically, Ribo-seq allowed for the identification of upstream open reading frames in transcripts, which we predict to be important regulators of translation.This method has the potential to (1) provide a new tool for studying cell-specific gene expression at the level of translation in tissues, (2) reveal new therapeutic targets to prevent cellular remodeling, and (3) trigger follow-up studies that address both, the molecular mechanisms involved in the posttranscriptional control of gene expression in cardiac cells, and the protective functions of proteins expressed in response to cellular stress.
LB  - PUB:(DE-HGF)16
C6  - pmid:31284834
DO  - DOI:10.1161/CIRCRESAHA.119.314817
UR  - https://inrepo02.dkfz.de/record/144532
ER  -