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@ARTICLE{Kiprianova:144748,
      author       = {I. Kiprianova$^*$ and A. Just$^*$ and B. Leuchs$^*$ and J.
                      Rommelaere$^*$ and A. L. Angelova$^*$},
      title        = {{F}luorescence {I}n {S}itu {H}ybridization ({FISH})
                      {D}etection of {V}iral {N}ucleic {A}cids in {O}ncolytic
                      {H}-1 {P}arvovirus-{T}reated {H}uman {B}rain {T}umors.},
      journal      = {Methods in molecular biology},
      volume       = {2058},
      issn         = {1064-3745},
      address      = {[Heidelberg]},
      publisher    = {[Springer]},
      reportid     = {DKFZ-2019-02180},
      isbn         = {978-1-4939-9793-0 (print)},
      pages        = {295-306},
      year         = {2020},
      note         = {#EA:F010#LA:F010#},
      abstract     = {Fluorescence in situ hybridization (FISH) is a specific,
                      sensitive, accurate, and reliable technique widely applied
                      in both research and clinic. Here we describe the detailed
                      protocol of a FISH method established by us to serve the
                      scientific purposes of the first oncolytic parvovirus
                      clinical trial (ParvOryx01). This trial was launched in
                      Germany in 2011. After trial completion in 2015, results
                      were published in Molecular Therapy in 2017. The primary
                      purpose of the trial was to evaluate the safety of an
                      oncolytic parvovirus, H-1PV (ParvOryx), in recurrent
                      glioblastoma patients. In addition, the efficiency of H-1PV
                      tumor targeting after intratumoral or systemic virus
                      administration was assessed by FISH detection of viral
                      nucleic acids (genomic single-stranded DNA, mRNA and
                      parvovirus double-stranded replicative forms) in
                      formalin-fixed paraffin-embedded glioblastoma tissues
                      resected at day 10 after ParvOryx treatment. The FISH method
                      allowed the detection-for the first time in humans-of H-1PV
                      replication markers in brain tumors of parvovirus-treated
                      patients. A protocol combining mRNA FISH with simultaneous
                      immunofluorescent staining for tumor and tumor
                      microenvironment markers was also developed and is described
                      here, in order to better characterize H-1PV cellular targets
                      and H-1PV treatment-associated tumor microenvironment
                      changes.},
      cin          = {F010},
      ddc          = {570},
      cid          = {I:(DE-He78)F010-20160331},
      pnm          = {316 - Infections and cancer (POF3-316)},
      pid          = {G:(DE-HGF)POF3-316},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)16},
      pubmed       = {pmid:31486047},
      doi          = {10.1007/978-1-4939-9794-7_20},
      url          = {https://inrepo02.dkfz.de/record/144748},
}