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@ARTICLE{Tisch:144870,
author = {N. Tisch and A. Freire-Valls and R. Yerbes and I. Paredes
and S. La Porta$^*$ and X. Wang and R. Martín-Pérez and L.
Castro and W. W. Wong and L. Coultas and B. Strilic and
H.-J. Gröne$^*$ and T. Hielscher$^*$ and C. Mogler and R.
Adams and P. Heiduschka and L. Claesson-Welsh and M. Mazzone
and A. López-Rivas and T. Schmidt and H. G. Augustin$^*$
and C. Ruiz de Almodovar},
title = {{C}aspase-8 modulates physiological and pathological
angiogenesis during retina development.},
journal = {The journal of clinical investigation},
volume = {129},
number = {12},
issn = {1558-8238},
address = {Ann Arbor, Mich.},
publisher = {ASCJ},
reportid = {DKFZ-2019-02294},
pages = {5092-5107},
year = {2019},
note = {2019 Dec 2;129(12):5092-5107},
abstract = {During developmental angiogenesis blood vessels grow and
remodel to ultimately build a hierarchical vascular network.
Whether and how cell death signaling molecules contribute to
blood vessel formation is still not well understood.
Caspase-8 (Casp-8), a key protease in the extrinsic cell
death-signaling pathway, regulates both cell death via
apoptosis and necroptosis. Here we show that expression of
Casp-8 in endothelial cells (ECs) was required for proper
postnatal retina angiogenesis. EC specific Casp-8 knockout
pups (Casp-8ECko) showed reduced retina angiogenesis, as the
loss of Casp-8 reduced EC proliferation, sprouting and
migration independent of its cell death function. Instead,
the loss of Casp-8 caused hyperactivation of p38
mitogen-activated protein kinase (MAPK) downstream of
receptor-interacting serine/threonine- protein kinase 3
(RIPK3) and destabilization of VE-cadherin at EC junctions.
In a mouse model of oxygen-induced retinopathy (OIR),
resembling retinopathy of prematurity (ROP), loss of Casp-8
in ECs was beneficial, as pathological neovascularization
was reduced in Casp-8ECko pups. Taken together, we describe
that Casp-8 acts in a cell-death independent manner in ECs
to regulate the formation of the retina vasculature and that
Casp-8 in ECs is mechanistically involved in the
pathophysiology of ROP.},
cin = {A190 / G130 / C060},
ddc = {610},
cid = {I:(DE-He78)A190-20160331 / I:(DE-He78)G130-20160331 /
I:(DE-He78)C060-20160331},
pnm = {319H - Addenda (POF3-319H)},
pid = {G:(DE-HGF)POF3-319H},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31454332},
doi = {10.1172/JCI122767},
url = {https://inrepo02.dkfz.de/record/144870},
}