TY  - JOUR
AU  - Bhardwaj, Megha
AU  - Weigl, Korbinian
AU  - Tikk, Kaja
AU  - Benner, Axel
AU  - Schrotz-King, Petra
AU  - Brenner, Hermann
TI  - Multiplex screening of 275 plasma protein biomarkers to identify a signature for early detection of colorectal cancer.
JO  - Molecular oncology
VL  - 14
IS  - 1
SN  - 1878-0261
CY  - Hoboken, NJ
PB  - John Wiley & Sons, Inc.
M1  - DKFZ-2019-02505
SP  - 8-21
PY  - 2020
N1  - 2020 Jan;14(1):8-21#EA:C120#LA:C070#
AB  - Blood-based protein biomarkers may be an attractive option for early detection of colorectal cancer (CRC). Here, we used a two-stage design to measure 275 protein markers by proximity extension assay (PEA), first in plasma samples of a discovery set consisting of 98 newly diagnosed CRC cases and 100 age and gender matched controls free of neoplasm at screening colonoscopy. An algorithm predicting the presence of early or late stage CRC was derived by Lasso regression with .632+ bootstrap method and the algorithms were then validated using PEA again in an independent validation set consisting of participants of screening colonoscopy with and without CRC (n= 56 and 102, respectively). Three different signatures for all, early, and late stages CRC consisting of 9, 12, and 11 protein markers were obtained in the discovery set with areas under the curves (AUCs) after .632+ bootstrap adjustment of 0.92, 0.91 and 0.96, respectively. External validation among participants of screening colonoscopy yielded AUCs of 0.76 (95
LB  - PUB:(DE-HGF)16
C6  - pmid:31652396
DO  - DOI:10.1002/1878-0261.12591
UR  - https://inrepo02.dkfz.de/record/147388
ER  -