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@ARTICLE{Ho:147392,
author = {C.-T. Ho$^*$ and T. Grousl$^*$ and O. Shatz$^*$ and A.
Jawed$^*$ and C. Ruger-Herreros$^*$ and M. Semmelink$^*$ and
R. Zahn$^*$ and K. Richter$^*$ and B. Bukau$^*$ and A.
Mogk$^*$},
title = {{C}ellular sequestrases maintain basal {H}sp70 capacity
ensuring balanced proteostasis.},
journal = {Nature Communications},
volume = {10},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {DKFZ-2019-02509},
pages = {4851},
year = {2019},
note = {DKFZ-ZMBH Alliance},
abstract = {Maintenance of cellular proteostasis is achieved by a
multi-layered quality control network, which counteracts the
accumulation of misfolded proteins by refolding and
degradation pathways. The organized sequestration of
misfolded proteins, actively promoted by cellular
sequestrases, represents a third strategy of quality
control. Here we determine the role of sequestration within
the proteostasis network in Saccharomyces cerevisiae and the
mechanism by which it occurs. The Hsp42 and Btn2
sequestrases are functionally intertwined with the refolding
activity of the Hsp70 system. Sequestration of misfolded
proteins by Hsp42 and Btn2 prevents proteostasis collapse
and viability loss in cells with limited Hsp70 capacity,
likely by shielding Hsp70 from misfolded protein overload.
Btn2 has chaperone and sequestrase activity and shares
features with small heat shock proteins. During stress
recovery Btn2 recruits the Hsp70-Hsp104 disaggregase by
directly interacting with the Hsp70 co-chaperone Sis1,
thereby shunting sequestered proteins to the refolding
pathway.},
cin = {A250 / W230},
ddc = {500},
cid = {I:(DE-He78)A250-20160331 / I:(DE-He78)W230-20160331},
pnm = {321 - Basic Concepts (POF3-321)},
pid = {G:(DE-HGF)POF3-321},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31649258},
pmc = {pmc:PMC6813348},
doi = {10.1038/s41467-019-12868-1},
url = {https://inrepo02.dkfz.de/record/147392},
}