000147608 001__ 147608
000147608 005__ 20240229123024.0
000147608 0247_ $$2doi$$a10.1007/s00441-019-03116-5
000147608 0247_ $$2pmid$$apmid:31713729
000147608 0247_ $$2ISSN$$a0044-3794
000147608 0247_ $$2ISSN$$a0302-766X
000147608 0247_ $$2ISSN$$a0340-0336
000147608 0247_ $$2ISSN$$a0373-031X
000147608 0247_ $$2ISSN$$a1432-0878
000147608 037__ $$aDKFZ-2019-02602
000147608 041__ $$aeng
000147608 082__ $$a610
000147608 1001_ $$0P:(DE-He78)7603e07539216b364cdb015f67872c66$$aFranke, Werner$$b0$$eFirst author$$udkfz
000147608 245__ $$aThe cell-cell junctions of mammalian testes. III. Absence of an endothelial cell layer covering the peritubular wall of the seminiferous tubules-an immunocytochemical correction of a 50-year-old error in the literature.
000147608 260__ $$aHeidelberg$$bSpringer$$c2020
000147608 3367_ $$2DRIVER$$aarticle
000147608 3367_ $$2DataCite$$aOutput Types/Journal article
000147608 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1601371226_26320
000147608 3367_ $$2BibTeX$$aARTICLE
000147608 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000147608 3367_ $$00$$2EndNote$$aJournal Article
000147608 500__ $$a2020 Jan;379(1):75-92#EA:A991#LA:A991#
000147608 520__ $$aIn the molecular biological and ultrastructural studies of the peritubular wall cells encasing the seminiferous tubules of mammalian testes, we found it necessary to characterize the outermost cell layer bordering on the interstitial space in detail. For half a century, the extremely thin cells of this monolayer have in the literature been regarded as part of a lymphatic endothelium, in particular in rodents. However, our double-label immunofluorescence microscopical results have shown that in all six mammalian species examined, including three rodent ones (rat, mouse, guinea pig), this classification is not correct: the very attenuated cells of this monolayer are not of lymphatic endothelial nature as they do not contain established endothelial marker molecules. In particular, they do not contain claudin-5-positive tight junctions, VE-cadherin-positive adherens junctions, 'lymph vessel endothelium hyaluronan receptor 1' (LYVE-1), podoplanin, protein myozap and 'von Willebrand Factor' (vWF). By contrast and as controls, all these established marker molecules for the lymphatic endothelial cell type are found in the endothelia of the lymph and-partly also-blood vessels located nearby in the interstitial space. Thus, our results provide evidence that the monolayer cells covering the peritubular wall do not contain endothelial marker molecules and hence are not endothelial cells. We discuss possible methodological reasons for the maintenance of this incorrect cell type classification in the literature and emphasize the value of molecular analyses using multiple cell type-specific markers, also with respect to physiology and medical sciences.
000147608 536__ $$0G:(DE-HGF)POF3-311$$a311 - Signalling pathways, cell and tumor biology (POF3-311)$$cPOF3-311$$fPOF III$$x0
000147608 588__ $$aDataset connected to CrossRef, PubMed,
000147608 7001_ $$0P:(DE-He78)d075d31409e4ebca72c76446442dbc1a$$aDomke, Lisa M$$b1$$udkfz
000147608 7001_ $$0P:(DE-He78)0f83789bc03d3966a9a786684c9b15e5$$aDörflinger, Yvette$$b2$$udkfz
000147608 7001_ $$0P:(DE-He78)7c58721b8a5cf92cb78cb3fad538ce3c$$aZimbelmann, Ralf$$b3$$eLast author$$udkfz
000147608 773__ $$0PERI:(DE-600)1458496-7$$a10.1007/s00441-019-03116-5$$n1$$p75-92$$tCell & tissue research$$v379$$x1432-0878$$y2020
000147608 909CO $$ooai:inrepo02.dkfz.de:147608$$pVDB
000147608 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)7603e07539216b364cdb015f67872c66$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000147608 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)d075d31409e4ebca72c76446442dbc1a$$aDeutsches Krebsforschungszentrum$$b1$$kDKFZ
000147608 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)0f83789bc03d3966a9a786684c9b15e5$$aDeutsches Krebsforschungszentrum$$b2$$kDKFZ
000147608 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)7c58721b8a5cf92cb78cb3fad538ce3c$$aDeutsches Krebsforschungszentrum$$b3$$kDKFZ
000147608 9131_ $$0G:(DE-HGF)POF3-311$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vSignalling pathways, cell and tumor biology$$x0
000147608 9141_ $$y2020
000147608 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000147608 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bCELL TISSUE RES : 2017
000147608 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000147608 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000147608 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000147608 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000147608 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000147608 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000147608 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000147608 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000147608 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000147608 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000147608 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record
000147608 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000147608 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000147608 9202_ $$0I:(DE-He78)A991-20160331$$kA991$$lA991 Helmholtz-Professur Zellbiologie$$x0
000147608 9200_ $$0I:(DE-He78)A991-20160331$$kA991$$lA991 Helmholtz-Professur Zellbiologie$$x0
000147608 9201_ $$0I:(DE-He78)A991-20160331$$kA991$$lA991 Helmholtz-Professur Zellbiologie$$x0
000147608 980__ $$ajournal
000147608 980__ $$aVDB
000147608 980__ $$aI:(DE-He78)A991-20160331
000147608 980__ $$aUNRESTRICTED