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@ARTICLE{Franke:147608,
      author       = {W. Franke$^*$ and L. M. Domke$^*$ and Y. Dörflinger$^*$
                      and R. Zimbelmann$^*$},
      title        = {{T}he cell-cell junctions of mammalian testes. {III}.
                      {A}bsence of an endothelial cell layer covering the
                      peritubular wall of the seminiferous tubules-an
                      immunocytochemical correction of a 50-year-old error in the
                      literature.},
      journal      = {Cell $\&$ tissue research},
      volume       = {379},
      number       = {1},
      issn         = {1432-0878},
      address      = {Heidelberg},
      publisher    = {Springer},
      reportid     = {DKFZ-2019-02602},
      pages        = {75-92},
      year         = {2020},
      note         = {2020 Jan;379(1):75-92#EA:A991#LA:A991#},
      abstract     = {In the molecular biological and ultrastructural studies of
                      the peritubular wall cells encasing the seminiferous tubules
                      of mammalian testes, we found it necessary to characterize
                      the outermost cell layer bordering on the interstitial space
                      in detail. For half a century, the extremely thin cells of
                      this monolayer have in the literature been regarded as part
                      of a lymphatic endothelium, in particular in rodents.
                      However, our double-label immunofluorescence microscopical
                      results have shown that in all six mammalian species
                      examined, including three rodent ones (rat, mouse, guinea
                      pig), this classification is not correct: the very
                      attenuated cells of this monolayer are not of lymphatic
                      endothelial nature as they do not contain established
                      endothelial marker molecules. In particular, they do not
                      contain claudin-5-positive tight junctions,
                      VE-cadherin-positive adherens junctions, 'lymph vessel
                      endothelium hyaluronan receptor 1' (LYVE-1), podoplanin,
                      protein myozap and 'von Willebrand Factor' (vWF). By
                      contrast and as controls, all these established marker
                      molecules for the lymphatic endothelial cell type are found
                      in the endothelia of the lymph and-partly also-blood vessels
                      located nearby in the interstitial space. Thus, our results
                      provide evidence that the monolayer cells covering the
                      peritubular wall do not contain endothelial marker molecules
                      and hence are not endothelial cells. We discuss possible
                      methodological reasons for the maintenance of this incorrect
                      cell type classification in the literature and emphasize the
                      value of molecular analyses using multiple cell
                      type-specific markers, also with respect to physiology and
                      medical sciences.},
      cin          = {A991},
      ddc          = {610},
      cid          = {I:(DE-He78)A991-20160331},
      pnm          = {311 - Signalling pathways, cell and tumor biology
                      (POF3-311)},
      pid          = {G:(DE-HGF)POF3-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31713729},
      doi          = {10.1007/s00441-019-03116-5},
      url          = {https://inrepo02.dkfz.de/record/147608},
}