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@ARTICLE{Ptasinska:147918,
author = {A. Ptasinska and A. Pickin and S. A. Assi and P. S. Chin
and L. Ames and R. Avellino and S. Gröschel$^*$ and R.
Delwel and P. N. Cockerill and C. S. Osborne and C. Bonifer},
title = {{RUNX}1-{ETO} {D}epletion in t(8;21) {AML} {L}eads to
{C}/{EBP}α- and {AP}-1-{M}ediated {A}lterations in
{E}nhancer-{P}romoter {I}nteraction.},
journal = {Cell reports},
volume = {28},
number = {12},
issn = {2211-1247},
address = {[New York, NY]},
publisher = {Elsevier},
reportid = {DKFZ-2019-02779},
pages = {3022 - 3031.e7},
year = {2019},
abstract = {Acute myeloid leukemia (AML) is associated with mutations
in transcriptional and epigenetic regulator genes impairing
myeloid differentiation. The t(8;21)(q22;q22) translocation
generates the RUNX1-ETO fusion protein, which interferes
with the hematopoietic master regulator RUNX1. We previously
showed that the maintenance of t(8;21) AML is dependent on
RUNX1-ETO expression. Its depletion causes extensive changes
in transcription factor binding, as well as gene expression,
and initiates myeloid differentiation. However, how these
processes are connected within a gene regulatory network is
unclear. To address this question, we performed
Promoter-Capture Hi-C assays, with or without RUNX1-ETO
depletion and assigned interacting cis-regulatory elements
to their respective genes. To construct a
RUNX1-ETO-dependent gene regulatory network maintaining AML,
we integrated cis-regulatory element interactions with gene
expression and transcription factor binding data. This
analysis shows that RUNX1-ETO participates
in cis-regulatory element interactions. However,
differential interactions following RUNX1-ETO depletion are
driven by alterations in the binding of RUNX1-ETO-regulated
transcription factors.},
cin = {A380},
ddc = {610},
cid = {I:(DE-He78)A380-20160331},
pnm = {311 - Signalling pathways, cell and tumor biology
(POF3-311)},
pid = {G:(DE-HGF)POF3-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31533028},
doi = {10.1016/j.celrep.2019.08.040},
url = {https://inrepo02.dkfz.de/record/147918},
}