%0 Journal Article
%A Braczynski, Anne K
%A Capper, David
%A Jones, David T W
%A Schittenhelm, Jens
%A Stichel, Damian
%A von Deimling, Andreas
%A Harter, Patrick
%A Mittelbronn, Michel
%T High density DNA methylation array is a reliable alternative for PCR-based analysis of the MGMT promoter methylation status in glioblastoma.
%J Pathology, research and practice
%V 216
%N 1
%@ 0344-0338
%C München
%I Elsevier
%M DKFZ-2019-02862
%P 152728
%D 2020
%Z Volume 216, Issue 1, January 2020, 152728
%X MGMT promoter methylation status is an important biomarker predicting survival and response to chemotherapy in patients suffering from glioblastoma. Since new diagnostic methods such as methylome-based classification of brain tumors are more and more frequently performed, we aimed at comparing the suitability of calculating the MGMT promoter methylation status in a quantitative manner from the methylome profiling as compared to the classic gold standard assessment by PCR.Our cohort consisted of 39 cases diagnosed as 'glioblastoma, IDH-wildtype' of which the MGMT promoter methylation status was analyzed with both methylation-specific PCR and high density DNA methylation array using the STP-27 algorithm. Contradictory results were validated by pyrosequencing.The inter-method reliability reached 77
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:31784096
%R 10.1016/j.prp.2019.152728
%U https://inrepo02.dkfz.de/record/148298