TY  - JOUR
AU  - Braczynski, Anne K
AU  - Capper, David
AU  - Jones, David T W
AU  - Schittenhelm, Jens
AU  - Stichel, Damian
AU  - von Deimling, Andreas
AU  - Harter, Patrick
AU  - Mittelbronn, Michel
TI  - High density DNA methylation array is a reliable alternative for PCR-based analysis of the MGMT promoter methylation status in glioblastoma.
JO  - Pathology, research and practice
VL  - 216
IS  - 1
SN  - 0344-0338
CY  - München
PB  - Elsevier
M1  - DKFZ-2019-02862
SP  - 152728
PY  - 2020
N1  - Volume 216, Issue 1, January 2020, 152728
AB  - MGMT promoter methylation status is an important biomarker predicting survival and response to chemotherapy in patients suffering from glioblastoma. Since new diagnostic methods such as methylome-based classification of brain tumors are more and more frequently performed, we aimed at comparing the suitability of calculating the MGMT promoter methylation status in a quantitative manner from the methylome profiling as compared to the classic gold standard assessment by PCR.Our cohort consisted of 39 cases diagnosed as 'glioblastoma, IDH-wildtype' of which the MGMT promoter methylation status was analyzed with both methylation-specific PCR and high density DNA methylation array using the STP-27 algorithm. Contradictory results were validated by pyrosequencing.The inter-method reliability reached 77
LB  - PUB:(DE-HGF)16
C6  - pmid:31784096
DO  - DOI:10.1016/j.prp.2019.152728
UR  - https://inrepo02.dkfz.de/record/148298
ER  -