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@ARTICLE{Weber:148382,
      author       = {S. Weber$^*$ and S. E. Koschade$^*$ and C. M. Hoffmann$^*$
                      and T. D. Dubash$^*$ and K. Giessler$^*$ and S. Dieter$^*$
                      and F. Herbst$^*$ and H. Glimm$^*$ and C. Ball$^*$},
      title        = {{T}he notch target gene {HEYL} modulates metastasis forming
                      capacity of colorectal cancer patient-derived spheroid cells
                      in vivo.},
      journal      = {BMC cancer},
      volume       = {19},
      number       = {1},
      issn         = {1471-2407},
      address      = {Heidelberg},
      publisher    = {Springer},
      reportid     = {DKFZ-2019-02938},
      pages        = {1181},
      year         = {2019},
      note         = {EA:L501LA:L301},
      abstract     = {While colorectal cancer (CRC) patients with localized
                      disease have a favorable prognosis, the five-year-survival
                      rate in patients with distant spread is still below $15\%.$
                      Hence, a detailed understanding of the mechanisms regulating
                      metastasis formation is essential to develop therapeutic
                      strategies targeting metastasized CRC. The notch pathway has
                      been shown to be involved in the metastatic spread of
                      various tumor entities; however, the impact of its target
                      gene HEYL remains unclear so far.In this study, we
                      functionally assessed the association between high HEYL
                      expression and metastasis formation in human CRC. Therefore,
                      we lentivirally overexpressed HEYL in two human
                      patient-derived CRC cultures differing in their spontaneous
                      metastasizing capacity and analyzed metastasis formation as
                      well as tumor cell dissemination into the bone marrow after
                      xenotransplantation into NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
                      (NSG) mice.HEYL overexpression decreased tumor cell
                      dissemination and the absolute numbers of formed metastases
                      in a sub-renal capsular spontaneous metastasis formation
                      model, addressing all steps of the metastatic cascade. In
                      contrast, metastatic capacity was not decreased following
                      intrasplenic xenotransplantation where the cells are placed
                      directly into the blood circulation.These results suggest
                      that HEYL negatively regulates metastasis formation in vivo
                      presumably by inhibiting intravasation of
                      metastasis-initiating cells.},
      cin          = {L501 / L301 / B280},
      ddc          = {610},
      cid          = {I:(DE-He78)L501-20160331 / I:(DE-He78)L301-20160331 /
                      I:(DE-He78)B280-20160331},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31796022},
      doi          = {10.1186/s12885-019-6396-4},
      url          = {https://inrepo02.dkfz.de/record/148382},
}