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@ARTICLE{Tarakanchikova:148726,
author = {Y. Tarakanchikova and J. Alzubi and V. Pennucci and M.
Follo and B. Kochergin and A. Muslimov and I. Skovorodkin
and S. Vainio and M. N. Antipina and V. Atkin and A. Popov
and I. Meglinski and T. Cathomen and T. I. Cornu and D. A.
Gorin and G. B. Sukhorukov and I. Nazarenko$^*$},
title = {{B}iodegradable {N}anocarriers {R}esembling {E}xtracellular
{V}esicles {D}eliver {G}enetic {M}aterial with the {H}ighest
{E}fficiency to {V}arious {C}ell {T}ypes.},
journal = {Small},
volume = {Dec},
issn = {1613-6829},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {DKFZ-2019-03252},
pages = {e1904880},
year = {2019},
note = {Small. 2019 Dec 16:e1904880},
abstract = {Efficient delivery of genetic material to primary cells
remains challenging. Here, efficient transfer of genetic
material is presented using synthetic biodegradable
nanocarriers, resembling extracellular vesicles in their
biomechanical properties. This is based on two main
technological achievements: generation of soft biodegradable
polyelectrolyte capsules in nanosize and efficient
application of the nanocapsules for co-transfer of different
RNAs to tumor cell lines and primary cells, including
hematopoietic progenitor cells and primary T cells. Near to
$100\%$ efficiency is reached using only 2.5 × 10-4 pmol of
siRNA, and 1 × 10-3 nmol of mRNA per cell, which is several
magnitude orders below the amounts reported for any of
methods published so far. The data show that biodegradable
nanocapsules represent a universal and highly efficient
biomimetic platform for the transfer of genetic material
with the utmost potential to revolutionize gene transfer
technology in vitro and in vivo.},
cin = {L601},
ddc = {540},
cid = {I:(DE-He78)L601-20160331},
pnm = {899 - ohne Topic (POF3-899)},
pid = {G:(DE-HGF)POF3-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31840408},
doi = {10.1002/smll.201904880},
url = {https://inrepo02.dkfz.de/record/148726},
}