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001     148828
005     20240229123035.0
024 7 _ |a 10.1038/s41556-019-0439-6
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024 7 _ |a 1465-7392
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024 7 _ |a 1476-4679
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037 _ _ |a DKFZ-2020-00020
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100 1 _ |a Baccin, Chiara
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245 _ _ |a Combined single-cell and spatial transcriptomics reveal the molecular, cellular and spatial bone marrow niche organization.
260 _ _ |a New York, NY
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|b Nature America
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520 _ _ |a The bone marrow constitutes the primary site for life-long blood production and skeletal regeneration. However, its cellular and spatial organization remains controversial. Here, we combine single-cell and spatially resolved transcriptomics to systematically map the molecular, cellular and spatial composition of distinct bone marrow niches. This allowed us to transcriptionally profile all major bone-marrow-resident cell types, determine their localization and clarify sources of pro-haematopoietic factors. Our data demonstrate that Cxcl12-abundant-reticular (CAR) cell subsets (Adipo-CAR and Osteo-CAR) differentially localize to sinusoidal and arteriolar surfaces, act locally as 'professional cytokine-secreting cells' and thereby establish peri-vascular micro-niches. Importantly, the three-dimensional bone-marrow organization can be accurately inferred from single-cell transcriptome data using the RNA-Magnet algorithm described here. Together, our study reveals the cellular and spatial organization of bone marrow niches and offers a systematic approach to dissect the complex organization of whole organs.
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700 1 _ |a Al-Sabah, Jude
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700 1 _ |a Velten, Lars
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700 1 _ |a Helbling, Patrick M
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700 1 _ |a Grünschläger, Florian
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700 1 _ |a Hernández-Malmierca, Pablo
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700 1 _ |a Nombela-Arrieta, César
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700 1 _ |a Steinmetz, Lars M
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700 1 _ |a Trumpp, Andreas
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700 1 _ |a Haas, Simon
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773 _ _ |a 10.1038/s41556-019-0439-6
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|v 22
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