%0 Journal Article
%A Liu, Junqi
%A Xue, Nannan
%A Guo, Yuexin
%A Niu, Kerun
%A Gao, Liang
%A Zhang, Song
%A Gu, Hao
%A Wang, Xin
%A Zhao, Di
%A Fan, Ruitai
%T CircRNA_100367 regulated the radiation sensitivity of esophageal squamous cell carcinomas through miR-217/Wnt3 pathway.315
%J Aging
%V 11
%N 24
%@ 1945-4589
%C [S.l.]
%I Impact Journals, LLC
%M DKFZ-2020-00236
%P 12412 - 12427
%D 2019
%X Circular RNAs (circRNAs) play important roles in regulating the radioresistance of esophageal squamous cell carcinoma (ESCC). This study aimed to determine the role of hsa_circRNA_100367 in regulating radioresistance of ESCC.Higher expression and potency of endothelial to mesenchymal transformation (EMT) was found in radioresistant ESCC cells (KYSE-150R) than in ESCC cells (KYSE-150). Silencing circRNA_100367 inhibited the proliferation and migration of KYSE-150R cells, and decreased the expression of β-catenin (an important molecule in Wnt pathway) in KYSE-150R cells. Additionally, circRNA_100367 bound to miR-217, and miR-217 targeted Wnt3. Low Wnt3 expression was associated with the short survival time in patients with ESCC and Wnt3 knockdown inhibited the proliferation and migration of KYSE-150R cells. CircRNA_100367 enhanced the radioresistance of KYSE-150R cells through miR-217/Wnt3 pathway. In vivo, circRNA_100367 silence reduced the growth of KYSE-150R cells under radiation.Our results revealed that circRNA_100367 attenuated radioresistance of ESCC through miR-217/Wnt3 pathway.CircRNAs related with the radioresistance of ESCC were analyzed by hierarchical cluster analysis. The relationship between circRNA_100367 and miR-217, Wnt3 was detected by RNA immunoprecipitation (RIP), RNA pull-down and luciferase reporte assays. The proliferation and migration ESCC cells were detected by MTT, Transwell and colony formation assays.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:31851619
%2 pmc:PMC6949088
%R 10.18632/aging.102580
%U https://inrepo02.dkfz.de/record/153183