TY  - JOUR
AU  - Bhardwaj, Megha
AU  - Weigl, Korbinian
AU  - Tikk, Kaja
AU  - Holland-Letz, Tim
AU  - Schrotz-King, Petra
AU  - Borchers, Christoph H
AU  - Brenner, Hermann
TI  - Multiplex quantitation of 270 plasma protein markers to identify a signature for early detection of colorectal cancer.
JO  - European journal of cancer
VL  - 127
SN  - 0959-8049
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - DKFZ-2020-00263
SP  - 30 - 40
PY  - 2020
N1  - #EA:C120#LA:C120#
AB  - Blood-based protein biomarker signatures might be an alternative or supplement to existing methods for early detection of colorectal cancer (CRC) for population-based screening. The objective of this study was to derive a protein biomarker signature for early detection of CRC and its precursor advanced adenoma (AA). In a two-stage design, 270 protein markers were measured by liquid chromatography/multiple reaction monitoring/mass spectrometry in plasma samples of discovery and validation sets. In the discovery set consisting of 100 newly diagnosed CRC cases and 100 age- and sex-matched controls free of neoplasm at screening colonoscopy, the algorithms predicting the presence of early- or late-stage CRC were derived by Lasso regression and .632 + bootstrap. The prediction algorithms were then externally validated in an independent validation set consisting of participants of screening colonoscopy including 56 participants with CRC, 99 with AA and 99 controls without any colorectal neoplasms. Three different signatures for all-, early- and late-stage CRC consisting of five-, three- and eight-protein markers were obtained in the discovery set with areas under the curves (AUCs) after .632 + bootstrap adjustment of 0.85, 0.83 and 0.96, respectively. External validation in the representative screening population yielded AUCs of 0.79 (95
LB  - PUB:(DE-HGF)16
C6  - pmid:31972396
DO  - DOI:10.1016/j.ejca.2019.11.021
UR  - https://inrepo02.dkfz.de/record/153230
ER  -