TY  - JOUR
AU  - Kim-Wanner, Soo-Zin
AU  - Assenov, Yassen
AU  - Nair, Mridul B
AU  - Weichenhan, Dieter
AU  - Benner, Axel
AU  - Becker, Natalia
AU  - Landwehr, Katharina
AU  - Kuner, Ruprecht
AU  - Sültmann, Holger
AU  - Esteller, Manel
AU  - Koch, Ina
AU  - Lindner, Michael
AU  - Meister, Michael
AU  - Thomas, Michael
AU  - Bieg, Matthias
AU  - Klingmueller, Ursula
AU  - Schlesner, Matthias
AU  - Warth, Arne
AU  - Brors, Benedikt
AU  - Seifried, Erhard
AU  - Bonig, Halvard
AU  - Plass, Christoph
AU  - Risch, Angela
AU  - Muley, Thomas
TI  - Genome-wide DNA methylation profiling in early stage I lung adenocarcinoma reveals predictive aberrant methylation in the promoter region of the long non-coding RNA PLUT - an exploratory study.
JO  - Journal of thoracic oncology
VL  - 15
IS  - 8
SN  - 1556-0864
CY  - Amsterdam
PB  - Elsevier
M1  - DKFZ-2020-00782
SP  - 1338-1350
PY  - 2020
N1  - 2020 Aug;15(8):1338-1350#EA:B370#
AB  - Surgery is the treatment of choice in early stage I lung adenocarcinoma. However, a considerable portion of patients experience recurrence within the first two years following complete resection. Especially suitable prognostic biomarkers are not available that identify patients at high risk for recurrence who may probably benefit from adjuvant treatment. This study aimed to identify methylation markers for early recurrence that may become important tools for the development of new treatment modalities.Genome-wide DNA methylation profiling was performed on 30 stage I lung adenocarcinomas comparing 14 patients with early metastatic recurrence to 16 patients with a long-term relapse-free survival period using methylated-CpG-immunoprecipitation (MCIp) followed by high-throughput next generation sequencing. The differentially methylated regions (DMRs) between the two subgroups were validated for their prognostic value in two independent cohorts using the MassCleave Assay, a high-resolution quantitative methylation analysis.Unsupervised clustering of patients in the discovery cohort based on DMRs identified patients with shorter relapse free survival (HR 2.23; 95
LB  - PUB:(DE-HGF)16
C6  - pmid:32272161
DO  - DOI:10.1016/j.jtho.2020.03.023
UR  - https://inrepo02.dkfz.de/record/154460
ER  -