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@ARTICLE{Hofmeister:154737,
      author       = {A. Hofmeister and M. C. Thomaßen and S. Markert and A.
                      Marquardt and M. Preußner and M. Rußwurm and R. T.
                      Schermuly and U. Steinhoff and H.-J. Gröne$^*$ and J. Hoyer
                      and B. D. Humphreys and I. Grgic},
      title        = {{D}evelopment of a new macrophage-specific {TRAP} mouse
                      ({M}ac{TRAP}) and definition of the renal macrophage
                      translational signature.},
      journal      = {Scientific reports},
      volume       = {10},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DKFZ-2020-00991},
      pages        = {7519},
      year         = {2020},
      abstract     = {Tissue macrophages play an important role in organ
                      homeostasis, immunity and the pathogenesis of various
                      inflammation-driven diseases. One major challenge has been
                      to selectively study resident macrophages in highly
                      heterogeneous organs such as kidney. To address this
                      problem, we adopted a Translational Ribosome Affinity
                      Purification (TRAP)- approach and designed a transgene that
                      expresses an eGFP-tagged ribosomal protein (L10a) under the
                      control of the macrophage-specific c-fms promoter to
                      generate c-fms-eGFP-L10a transgenic mice (MacTRAP). Rigorous
                      characterization found no gross abnormalities in MacTRAP
                      mice and confirmed transgene expression across various
                      organs. Immunohistological analyses of MacTRAP kidneys
                      identified eGFP-L10a expressing cells in the
                      tubulointerstitial compartment which stained positive for
                      macrophage marker F4/80. Inflammatory challenge led to
                      robust eGFP-L10a upregulation in kidney, confirming MacTRAP
                      responsiveness in vivo. We successfully extracted
                      macrophage-specific polysomal RNA from MacTRAP kidneys and
                      conducted RNA sequencing followed by bioinformatical
                      analyses, hereby establishing a comprehensive and unique in
                      vivo gene expression and pathway signature of resident renal
                      macrophages. In summary, we created, validated and applied a
                      new, responsive macrophage-specific TRAP mouse line,
                      defining the translational profile of renal macrophages and
                      dendritic cells. This new tool may be of great value for the
                      study of macrophage biology in different organs and various
                      models of injury and disease.},
      cin          = {G130},
      ddc          = {600},
      cid          = {I:(DE-He78)G130-20160331},
      pnm          = {319H - Addenda (POF3-319H)},
      pid          = {G:(DE-HGF)POF3-319H},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32372032},
      doi          = {10.1038/s41598-020-63514-6},
      url          = {https://inrepo02.dkfz.de/record/154737},
}