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@ARTICLE{Weber:156972,
author = {J. Weber and C. J. Braun$^*$ and D. Saur$^*$ and R.
Rad$^*$},
title = {{I}n vivo functional screening for systems-level
integrative cancer genomics.},
journal = {Nature reviews / Cancer Cancer},
volume = {20},
number = {10},
issn = {1474-1768},
address = {London [u.a.]},
publisher = {Nature Publ. Group},
reportid = {DKFZ-2020-01277},
pages = {573-593},
year = {2020},
note = {2020 Oct;20(10):573-593},
abstract = {With the genetic portraits of all major human malignancies
now available, we next face the challenge of characterizing
the function of mutated genes, their downstream targets,
interactions and molecular networks. Moreover, poorly
understood at the functional level are also non-mutated but
dysregulated genomes, epigenomes or transcriptomes.
Breakthroughs in manipulative mouse genetics offer new
opportunities to probe the interplay of molecules, cells and
systemic signals underlying disease pathogenesis in higher
organisms. Herein, we review functional screening strategies
in mice using genetic perturbation and chemical mutagenesis.
We outline the spectrum of genetic tools that exist, such as
transposons, CRISPR and RNAi and describe discoveries
emerging from their use. Genome-wide or targeted screens are
being used to uncover genomic and regulatory landscapes in
oncogenesis, metastasis or drug resistance. Versatile
screening systems support experimentation in diverse genetic
and spatio-temporal settings to integrate molecular,
cellular or environmental context-dependencies. We also
review the combination of in vivo screening and barcoding
strategies to study genetic interactions and quantitative
cancer dynamics during tumour evolution. These scalable
functional genomics approaches are transforming our ability
to interrogate complex biological systems.},
subtyp = {Review Article},
cin = {B062 / MU01},
ddc = {610},
cid = {I:(DE-He78)B062-20160331 / I:(DE-He78)MU01-20160331},
pnm = {315 - Imaging and radiooncology (POF3-315)},
pid = {G:(DE-HGF)POF3-315},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32636489},
doi = {10.1038/s41568-020-0275-9},
url = {https://inrepo02.dkfz.de/record/156972},
}