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000157014 0247_ $$2doi$$a10.1016/j.isci.2020.101127
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000157014 1001_ $$0P:(DE-He78)a4340add02b610f9dbdba2dc909cbd09$$aDelacher, Michael$$b0$$eFirst author
000157014 245__ $$aQuantitative Proteomics Identifies TCF1 as a Negative Regulator of Foxp3 Expression in Conventional T Cells.
000157014 260__ $$aSt. Louis$$bElsevier$$c2020
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000157014 520__ $$aRegulatory T cells are important regulators of the immune system and have versatile functions for the homeostasis and repair of tissues. They express the forkhead box transcription factor Foxp3 as a lineage-defining protein. Negative regulators of Foxp3 expression are not well understood. Here, we generated double-stranded DNA probes complementary to the Foxp3 promoter sequence and performed a pull-down with nuclear protein in vitro, followed by elution of bound proteins and quantitative mass spectrometry. Of the Foxp3-promoter-binding transcription factors identified with this approach, one was T cell factor 1 (TCF1). Using viral over-expression, we identified TCF1 as a repressor of Foxp3 expression. In TCF1-deficient animals, increased levels of Foxp3intermediateCD25negative T cells were identified. CRISPR-Cas9 knockout studies in primary human and mouse conventional CD4 T (Tconv) cells revealed that TCF1 protects Tconv cells from inadvertent Foxp3 expression. Our data implicate a role of TCF1 in suppressing Foxp3 expression in activated T cells.
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000157014 7001_ $$0P:(DE-HGF)0$$aBarra, Melanie M$$b1
000157014 7001_ $$aHerzig, Yonatan$$b2
000157014 7001_ $$aEichelbaum, Katrin$$b3
000157014 7001_ $$aRafiee, Mahmoud-Reza$$b4
000157014 7001_ $$0P:(DE-HGF)0$$aRichards, David M$$b5
000157014 7001_ $$0P:(DE-He78)4d320583e7adaedcb8374d787eb6bfe5$$aTräger, Ulrike$$b6
000157014 7001_ $$0P:(DE-He78)24f65e08387eafe8f511b710e366fdf4$$aHofer, Ann-Cathrin$$b7
000157014 7001_ $$0P:(DE-HGF)0$$aKazakov, Alexander$$b8
000157014 7001_ $$0P:(DE-He78)48a0f3d6278cc8d51ce937ca7db9dba8$$aBraband, Kathrin$$b9
000157014 7001_ $$0P:(DE-HGF)0$$aGonzalez, Marina$$b10
000157014 7001_ $$aWöhrl, Lukas$$b11
000157014 7001_ $$aSchambeck, Kathrin$$b12
000157014 7001_ $$0P:(DE-HGF)0$$aImbusch, Charles D$$b13
000157014 7001_ $$aAbramson, Jakub$$b14
000157014 7001_ $$0P:(DE-He78)939d5891259c638c1ab053b1456a578c$$aKrijgsveld, Jeroen$$b15
000157014 7001_ $$0P:(DE-He78)f0638edf1d3f61c0d7ebb587f511733f$$aFeuerer, Markus$$b16$$eLast author
000157014 773__ $$0PERI:(DE-600)2927064-9$$a10.1016/j.isci.2020.101127$$gVol. 23, no. 5, p. 101127 -$$n5$$p101127$$tiScience$$v23$$x2589-0042$$y2020
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