%0 Journal Article
%A Tsang, Sabrina H
%A Basu, Partha
%A Bender, Noemi
%A Herrero, Rolando
%A Kemp, Troy J
%A Kreimer, Aimée R
%A Müller, Martin
%A Panicker, Gitika
%A Pawlita, Michael
%A Pinto, Ligia A
%A Sampson, Joshua N
%A Sankaranarayanan, Rengaswamy
%A Schussler, John
%A Sehr, Peter
%A Sierra, Monica S
%A Unger, Elizabeth R
%A Waterboer, Tim
%A Hildesheim, Allan
%T Evaluation of serological assays to monitor antibody responses to single-dose HPV vaccines.
%J Vaccine
%V 38
%N 38
%@ 0264-410X
%C Amsterdam
%I Elsevier
%M DKFZ-2020-01504
%P 5997-6006
%D 2020
%Z 2020 Aug 27;38(38):5997-6006
%X Whether existing serological assays are sufficiently robust to measure the lower antibody levels expected following single-dose HPV vaccination is unknown.We evaluated seven assays measuring HPV-16/18 immunological responses overall and by number of doses in 530 serum samples from participants receiving varying doses of Cervarix or Gardasil up to 36-months post-vaccination. Serum was evaluated by simplex (HPV-16 ELISA, HPV-18 ELISA), multiplex (LIA-4, VLP-MIA, M9ELISA, GST-L1), and high-throughput pseudovirion-based neutralization assays (HT-PBNA), and results were compared to the gold standard HPV-16/18 secreted alkaline phosphatase neutralization assay (SEAP-NA). Reproducibility was assessed by the coefficient of variation (CV) and intraclass correlation coefficient (ICC). Percent agreement, Pearson correlation, and weighted-kappa were used to assess validity. Determinants of seronegativity were evaluated by chi-squared test.HPV-16: Seropositivity range was 97.1-99.5
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:32713678
%R 10.1016/j.vaccine.2020.07.017
%U https://inrepo02.dkfz.de/record/157247