TY  - JOUR
AU  - Tsang, Sabrina H
AU  - Basu, Partha
AU  - Bender, Noemi
AU  - Herrero, Rolando
AU  - Kemp, Troy J
AU  - Kreimer, Aimée R
AU  - Müller, Martin
AU  - Panicker, Gitika
AU  - Pawlita, Michael
AU  - Pinto, Ligia A
AU  - Sampson, Joshua N
AU  - Sankaranarayanan, Rengaswamy
AU  - Schussler, John
AU  - Sehr, Peter
AU  - Sierra, Monica S
AU  - Unger, Elizabeth R
AU  - Waterboer, Tim
AU  - Hildesheim, Allan
TI  - Evaluation of serological assays to monitor antibody responses to single-dose HPV vaccines.
JO  - Vaccine
VL  - 38
IS  - 38
SN  - 0264-410X
CY  - Amsterdam
PB  - Elsevier
M1  - DKFZ-2020-01504
SP  - 5997-6006
PY  - 2020
N1  - 2020 Aug 27;38(38):5997-6006
AB  - Whether existing serological assays are sufficiently robust to measure the lower antibody levels expected following single-dose HPV vaccination is unknown.We evaluated seven assays measuring HPV-16/18 immunological responses overall and by number of doses in 530 serum samples from participants receiving varying doses of Cervarix or Gardasil up to 36-months post-vaccination. Serum was evaluated by simplex (HPV-16 ELISA, HPV-18 ELISA), multiplex (LIA-4, VLP-MIA, M9ELISA, GST-L1), and high-throughput pseudovirion-based neutralization assays (HT-PBNA), and results were compared to the gold standard HPV-16/18 secreted alkaline phosphatase neutralization assay (SEAP-NA). Reproducibility was assessed by the coefficient of variation (CV) and intraclass correlation coefficient (ICC). Percent agreement, Pearson correlation, and weighted-kappa were used to assess validity. Determinants of seronegativity were evaluated by chi-squared test.HPV-16: Seropositivity range was 97.1-99.5
LB  - PUB:(DE-HGF)16
C6  - pmid:32713678
DO  - DOI:10.1016/j.vaccine.2020.07.017
UR  - https://inrepo02.dkfz.de/record/157247
ER  -