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@ARTICLE{Kolb:157333,
      author       = {T. Kolb$^*$ and U. Khalid$^*$ and M. Simović$^*$ and M.
                      Ratnaparkhe$^*$ and J. Wong$^*$ and A. Jauch and P.
                      Schmezer$^*$ and A. Rode$^*$ and S. Sebban and D. Haag$^*$
                      and M. Hergt$^*$ and F. Devens$^*$ and Y. Buganim and M.
                      Zapatka$^*$ and P. Lichter$^*$ and A. Ernst$^*$},
      title        = {{A} versatile system to introduce clusters of genomic
                      double-strand breaks in large cell populations.},
      journal      = {Genes, chromosomes $\&$ cancer},
      volume       = {60},
      number       = {5},
      issn         = {1098-2264},
      address      = {New York, NY},
      publisher    = {Wiley-Liss},
      reportid     = {DKFZ-2020-01562},
      pages        = {303-313},
      year         = {2021},
      note         = {#EA:B420#LA:B420#2021 May;60(5):303-313},
      abstract     = {In vitro assays for clustered DNA lesions will facilitate
                      the analysis of the mechanisms underlying complex genome
                      rearrangements such as chromothripsis, including the
                      recruitment of repair factors to sites of DNA double-strand
                      breaks. We present a novel method generating localized DNA
                      double-strand breaks using UV-irradiation with photomasks.
                      The size of the damage foci and the spacing between lesions
                      are fully adjustable, making the assay suitable for
                      different cell types and targeted areas. We validated this
                      set-up with genomically stable epithelial cells, normal
                      fibroblasts, pluripotent stem cells and patient-derived
                      primary cultures. Our method does not require a specialized
                      device such as a laser, making it accessible to a broad
                      range of users. Sensitization by BrdU incorporation is not
                      required, which enables analyzing the DNA damage response in
                      post-mitotic cells. Irradiated cells can be cultivated
                      further, followed by time-lapse imaging or used for
                      downstream biochemical analyses, thanks to the
                      high-throughput of the system. Importantly, we showed genome
                      rearrangements in the irradiated cells, providing a proof of
                      principle for the induction of structural variants by
                      localized DNA lesions. This article is protected by
                      copyright. All rights reserved.},
      cin          = {B420 / HD01 / B370 / B062 / B060},
      ddc          = {610},
      cid          = {I:(DE-He78)B420-20160331 / I:(DE-He78)HD01-20160331 /
                      I:(DE-He78)B370-20160331 / I:(DE-He78)B062-20160331 /
                      I:(DE-He78)B060-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32734664},
      doi          = {10.1002/gcc.22890},
      url          = {https://inrepo02.dkfz.de/record/157333},
}