000157427 001__ 157427
000157427 005__ 20240229123138.0
000157427 0247_ $$2doi$$a10.1093/nar/gkaa459
000157427 0247_ $$2pmid$$apmid:32479629
000157427 0247_ $$2pmc$$apmc:PMC7367185
000157427 0247_ $$2ISSN$$a0305-1048
000157427 0247_ $$2ISSN$$a0309-1872
000157427 0247_ $$2ISSN$$a1362-4954
000157427 0247_ $$2ISSN$$a1362-4962
000157427 0247_ $$2altmetric$$aaltmetric:83269054
000157427 037__ $$aDKFZ-2020-01622
000157427 041__ $$aeng
000157427 082__ $$a570
000157427 1001_ $$aBecker, Martin$$b0
000157427 245__ $$aCLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries.
000157427 260__ $$aOxford$$bOxford Univ. Press$$c2020
000157427 3367_ $$2DRIVER$$aarticle
000157427 3367_ $$2DataCite$$aOutput Types/Journal article
000157427 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1600154794_32125
000157427 3367_ $$2BibTeX$$aARTICLE
000157427 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000157427 3367_ $$00$$2EndNote$$aJournal Article
000157427 500__ $$a#LA:B062#
000157427 520__ $$aThe systematic perturbation of genomes using CRISPR/Cas9 deciphers gene function at an unprecedented rate, depth and ease. Commercially available sgRNA libraries typically contain tens of thousands of pre-defined constructs, resulting in a complexity challenging to handle. In contrast, custom sgRNA libraries comprise gene sets of self-defined content and size, facilitating experiments under complex conditions such as in vivo systems. To streamline and upscale cloning of custom libraries, we present CLUE, a bioinformatic and wet-lab pipeline for the multiplexed generation of pooled sgRNA libraries. CLUE starts from lists of genes or pasted sequences provided by the user and designs a single synthetic oligonucleotide pool containing various libraries. At the core of the approach, a barcoding strategy for unique primer binding sites allows amplifying different user-defined libraries from one single oligonucleotide pool. We prove the approach to be straightforward, versatile and specific, yielding uniform sgRNA distributions in all resulting libraries, virtually devoid of cross-contaminations. For in silico library multiplexing and design, we established an easy-to-use online platform at www.crispr-clue.de. All in all, CLUE represents a resource-saving approach to produce numerous high quality custom sgRNA libraries in parallel, which will foster their broad use across molecular biosciences.
000157427 536__ $$0G:(DE-HGF)POF3-312$$a312 - Functional and structural genomics (POF3-312)$$cPOF3-312$$fPOF III$$x0
000157427 588__ $$aDataset connected to CrossRef, PubMed,
000157427 7001_ $$aNoll-Puchta, Heidi$$b1
000157427 7001_ $$aAmend, Diana$$b2
000157427 7001_ $$aNolte, Florian$$b3
000157427 7001_ $$aFuchs, Christiane$$b4
000157427 7001_ $$0P:(DE-He78)0dc58a3349e4e66a46410a2067e43828$$aJeremias, Irmela$$b5$$udkfz
000157427 7001_ $$0P:(DE-He78)19484c34bd0a338b52b4c22786721676$$aBraun, Christian Jörg$$b6$$eLast author$$udkfz
000157427 773__ $$0PERI:(DE-600)1472175-2$$a10.1093/nar/gkaa459$$gVol. 48, no. 13, p. gkaa459$$n13$$pe78$$tNucleic acids research$$v48$$x0301-5610$$y2020
000157427 909CO $$ooai:inrepo02.dkfz.de:157427$$pVDB
000157427 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)0dc58a3349e4e66a46410a2067e43828$$aDeutsches Krebsforschungszentrum$$b5$$kDKFZ
000157427 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)19484c34bd0a338b52b4c22786721676$$aDeutsches Krebsforschungszentrum$$b6$$kDKFZ
000157427 9131_ $$0G:(DE-HGF)POF3-312$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vFunctional and structural genomics$$x0
000157427 9141_ $$y2020
000157427 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2020-01-16$$wger
000157427 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNUCLEIC ACIDS RES : 2018$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Peer review$$d2020-01-16
000157427 915__ $$0LIC:(DE-HGF)CCBYNCNV$$2V:(DE-HGF)$$aCreative Commons Attribution-NonCommercial CC BY-NC (No Version)$$bDOAJ$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)9910$$2StatID$$aIF >= 10$$bNUCLEIC ACIDS RES : 2018$$d2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$f2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$f2020-01-16
000157427 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2020-01-16
000157427 9202_ $$0I:(DE-He78)B062-20160331$$kB062$$lB062 Pädiatrische Neuroonkologie$$x0
000157427 9201_ $$0I:(DE-He78)MU01-20160331$$kMU01$$lDKTK MU LMU zentral$$x0
000157427 9201_ $$0I:(DE-He78)B062-20160331$$kB062$$lB062 Pädiatrische Neuroonkologie$$x1
000157427 980__ $$ajournal
000157427 980__ $$aVDB
000157427 980__ $$aI:(DE-He78)MU01-20160331
000157427 980__ $$aI:(DE-He78)B062-20160331
000157427 980__ $$aUNRESTRICTED