TY  - JOUR
AU  - Ecker, Jonas
AU  - Thatikonda, Venu
AU  - Sigismondo, Gianluca
AU  - Selt, Florian
AU  - Valinciute, Gintvile
AU  - Oehme, Ina
AU  - Müller, Carina
AU  - Buhl, Juliane L
AU  - Ridinger, Johannes
AU  - Usta, Diren
AU  - Qin, Nan
AU  - van Tilburg, Cornelis M
AU  - Herold-Mende, Christel
AU  - Remke, Marc
AU  - Sahm, Felix
AU  - Westermann, Frank
AU  - Kool, Marcel
AU  - Wechsler-Reya, Robert J
AU  - Chavez, Lukas
AU  - Krijgsveld, Jeroen
AU  - Jäger, Natalie
AU  - Pfister, Stefan M
AU  - Witt, Olaf
AU  - Milde, Till
TI  - Reduced chromatin binding of MYC is a key effect of HDAC inhibition in MYC amplified medulloblastoma.
JO  - Neuro-Oncology
VL  - 23
IS  - 2
SN  - 1523-5866
CY  - Oxford
PB  - Oxford Univ. Press
M1  - DKFZ-2020-01721
SP  - 226-239
PY  - 2021
N1  - #EA:B310#LA:B310#2021 Feb 25;23(2):226-239
AB  - The sensitivity of MYC amplified medulloblastoma to class I HDAC inhibition has been shown previously, however understanding the underlying molecular mechanism is crucial for selection of effective HDAC inhibitors for clinical use. The aim of this study was to investigate the direct molecular interaction of MYC and the class I HDAC2, and the impact of class I HDAC inhibition on MYC function.Co-immunoprecipitation and mass spectrometry was used to determine the co-localization of MYC and HDAC2. ChIP-sequencing and gene expression profiling was used to analyze the co- localization of MYC and HDAC2 on DNA and the impact on transcriptional activity in primary tumors and a MYC amplified cell line treated with the class I HDAC inhibitor entinostat. The effect on MYC was investigated by quantitative RT-PCR, Western blot and immunofluorescence.HDAC2 is a cofactor of MYC in MYC amplified medulloblastoma. The MYC-HDAC2 complex is bound to genes defining the MYC-dependent transcriptional profile. Class I HDAC inhibition leads to stabilization and reduced DNA-binding of MYC protein inducing a down-regulation of MYC activated genes (MAGs) and up-regulation of MYC repressed genes (MRGs). MAGs and MRGs are characterized by opposing biological functions and by distinct E-box distribution.Our data elucidates the molecular interaction of MYC and HDAC2 and support a model in which inhibition of class I HDACs directly targets MYC´s trans-activating and trans-repressing function.
LB  - PUB:(DE-HGF)16
C6  - pmid:32822486
DO  - DOI:10.1093/neuonc/noaa191
UR  - https://inrepo02.dkfz.de/record/157624
ER  -