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@ARTICLE{Chen:157750,
      author       = {I.-P. Chen and M. Bender and I. Spassova$^*$ and S. Henning
                      and L. Kubat$^*$ and K. Fan$^*$ and S. Degenhardt and M.
                      Mhamdi-Ghodbani and A. Sriram$^*$ and B. Volkmer and P.
                      Boukamp$^*$ and J. C. Becker$^*$ and R. Greinert},
      title        = {{UV}-type specific alteration of mi{RNA} expression and its
                      association with tumor progression and metastasis in {SCC}
                      cell lines.},
      journal      = {Journal of cancer research and clinical oncology},
      volume       = {146},
      number       = {12},
      issn         = {0171-5216},
      address      = {Berlin},
      publisher    = {Springer42162},
      reportid     = {DKFZ-2020-01787},
      pages        = {3215-3231},
      year         = {2020},
      note         = {2020 Dec;146(12):3215-3231},
      abstract     = {UV exposure is the main risk factor for development of
                      cutaneous squamous cell carcinoma (cSCC). While early
                      detection greatly improves cSCC prognosis, locally advanced
                      or metastatic cSCC has a severely impaired prognosis.
                      Notably, the mechanisms of progression to metastatic cSCC
                      are not well understood. We hypothesized that UV exposure of
                      already transformed epithelial cSCC cells further induces
                      changes which might be involved in the progression to
                      metastatic cSCCs and that UV-inducible microRNAs (miRNAs)
                      might play an important role.Thus, we analyzed the impact of
                      UV radiation of different quality (UVA, UVB, UVA + UVB)
                      on the miRNA expression pattern in established cell lines
                      generated from primary and metastatic cSCCs (Met-1, Met-4)
                      using the NanoString nCounter platform.This analysis
                      revealed that the expression pattern of miRNAs depends on
                      both the cell line used per se and on the quality of UV
                      radiation. Comparison of UV-induced miRNAs in cSCC cell
                      lines established from a primary tumor (Met-1) and the
                      respective (un-irradiated) metastasis (Met-4) suggest that
                      miR-7-5p, miR-29a-3p and miR-183-5p are involved in a
                      UV-driven pathway of progression to metastasis. This notion
                      is supported by the fact that these three miRNAs build up a
                      network of 81 potential target genes involved e.g. in
                      UVA/UVB-induced MAPK signaling and regulation of the
                      epithelial-mesenchymal transition. As an example, PTEN, a
                      target of UV-upregulated miRNAs (miR-29a-3p, miR-183-5p),
                      could be shown to be down-regulated in response to UV
                      radiation. We further identified CNOT8, the transcription
                      complex subunit 8 of the CCR4-NOT complex, a deadenylase
                      removing the poly(A) tail from miRNA-destabilized mRNAs, in
                      the center of this network, targeted by all three miRNAs.In
                      summary, our results demonstrate that UV radiation induces
                      an miRNA expression pattern in primary SCC cell line partly
                      resembling those of metastatic cell line, thus suggesting
                      that UV radiation impacts SCC progression beyond
                      initiation.},
      cin          = {ED01 / A110},
      ddc          = {610},
      cid          = {I:(DE-He78)ED01-20160331 / I:(DE-He78)A110-20160331},
      pnm          = {311 - Signalling pathways, cell and tumor biology
                      (POF3-311)},
      pid          = {G:(DE-HGF)POF3-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32865618},
      doi          = {10.1007/s00432-020-03358-9},
      url          = {https://inrepo02.dkfz.de/record/157750},
}