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@ARTICLE{Zhang:163953,
author = {Z. Zhang and J. W. Bartsch and J. Benzel$^*$ and T. Lei and
C. Nimsky and B. Voellger},
title = {{S}elective estrogen receptor modulators decrease
invasiveness in pituitary adenoma cell lines {A}t{T}-20 and
{T}t{T}/{GF} by affecting expression of {MMP}-14 and
{ADAM}12.},
journal = {FEBS Open Bio},
volume = {10},
number = {11},
issn = {2211-5463},
address = {Cambridge},
publisher = {Elsevier on behalf of the Federation of European
Biochemical Societies},
reportid = {DKFZ-2020-02166},
pages = {2489-2498},
year = {2020},
note = {2020 Oct 8;10(11):2489-2498},
abstract = {Selective estrogen receptor modulators (SERMs)
significantly affect survival and invasiveness of rodent
pituitary adenoma (PA) cells. The impact of three clinically
relevant SERMs (bazedoxifene, clomiphene, raloxifene) on
invasiveness and on gene and protein expression of
invasion-related proteases (Matrix Metalloproteinase-14
(MMP-14) and A Disintegrin And Metalloproteinase-12
(ADAM12)) was analyzed in murine PA cells (AtT-20 and
TtT/GF). All SERMs significantly decreased cell
invasiveness. Moreover, SERMs significantly decreased
expression of ADAM12 mRNA in both cell lines and of MMP-14
mRNA in TtT/GF cells. Invasion rates of AtT-20 and TtT/GF
significantly decreased after ADAM12 gene silencing, and the
invasion rate of TtT/GF cells significantly decreased after
MMP-14 gene silencing. All SERMs affected ADAM12 protein
expression in AtT-20 cells whereas bazedoxifene and
raloxifene decreased MMP-14 protein expression in TtT/GF
cells. We conclude that SERMs attenuate invasiveness of
murine PA cells by downregulating expression levels of
invasion-related proteases MMP-14 and ADAM12.},
cin = {B062},
ddc = {570},
cid = {I:(DE-He78)B062-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33030286},
doi = {10.1002/2211-5463.12999},
url = {https://inrepo02.dkfz.de/record/163953},
}