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@ARTICLE{Rusert:163998,
author = {J. M. Rusert and E. F. Juarez and S. Brabetz$^*$ and J.
Jensen and A. Garancher and L. Q. Chau and S. K.
Tacheva-Grigorova and S. Wahab and Y. T. Udaka and D. Finlay
and H. Seker-Cin$^*$ and B. Reardon and S. Gröbner$^*$ and
J. Serrano and J. Ecker$^*$ and L. Qi and M. Kogiso and Y.
Du and P. A. Baxter and J. J. Henderson and M. E. Berens and
K. Vuori and T. Milde$^*$ and Y.-J. Cho and X.-N. Li and J.
M. Olson and I. Reyes and M. Snuderl and T. C. Wong and D.
P. Dimmock and S. A. Nahas and D. Malicki and J. R. Crawford
and M. L. Levy and E. M. Van Allen and S. Pfister$^*$ and P.
Tamayo and M. Kool$^*$ and J. P. Mesirov and R. J.
Wechsler-Reya},
title = {{F}unctional precision medicine identifies new therapeutic
candidates for medulloblastoma.},
journal = {Cancer research},
volume = {80},
number = {23},
issn = {1538-7445},
address = {Philadelphia, Pa.},
publisher = {AACR},
reportid = {DKFZ-2020-02198},
pages = {5393-5407},
year = {2020},
note = {2020 Dec 1;80(23):5393-5407},
abstract = {Medulloblastoma (MB) is among the most common malignant
brain tumors in children. Recent studies have identified at
least four subgroups of the disease that differ in terms of
molecular characteristics and patient outcomes. Despite this
heterogeneity, most MB patients receive similar therapies,
including surgery, radiation, and intensive chemotherapy.
Although these treatments prolong survival, many patients
still die from the disease and survivors suffer severe
long-term side effects from therapy. We hypothesize that
each MB patient is sensitive to different therapies and that
tailoring therapy based on the molecular and cellular
characteristics of patient tumors will improve outcomes. To
test this, we assembled a panel of orthotopic
patient-derived xenografts (PDX) and subjected them to DNA
sequencing, gene expression profiling, and high-throughput
drug screening. Analysis of DNA sequencing revealed that
most MB do not have actionable mutations that point to
effective therapies. In contrast, gene expression and drug
response data provided valuable information about potential
therapies for every tumor. For example, drug screening
demonstrated that actinomycin D, which is used for treatment
of sarcoma but rarely for MB, was active against PDX
representing Group 3 MB, the most aggressive form of the
disease. Functional analysis of tumor cells was successfully
used in a clinical setting to identify more treatment
options than sequencing alone. These studies suggest that it
should be possible to move away from a one-size-fits-all
approach and begin to treat each patient with therapies that
are effective against their specific tumor.},
cin = {B062 / HD01 / B310},
ddc = {610},
cid = {I:(DE-He78)B062-20160331 / I:(DE-He78)HD01-20160331 /
I:(DE-He78)B310-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33046443},
doi = {10.1158/0008-5472.CAN-20-1655},
url = {https://inrepo02.dkfz.de/record/163998},
}